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一种新的酶结晶方法.pdf

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34 1 Vol. 34 No. 1 2006 1 CHEM ICAL ENGINEERING (CH INA) Jan. 2006 刘均洪, 邱龙辉, 曹红光, 张媛媛, 曹树煜, 李燕妮 ( , 266042) : , , , 40 30 , , , ,, , : ,, : ;; : Q 551 : A : 2006) A novel crystallizationmethod of soybean hullperoxidase LIU Junhong, QIU Longhu,i CAO ongguang, Z ANG Yuanyuan, CAO Shuyu, LI Yanni (Depart ent ofB ioengineering Phar aceutics, Q ingdaoUniversity ofScience and Technology, Q ingdao 266042, ShandongProvince, China) Abstract: Soybean hullperoxides ( SHP) was crystallizedw ith enzy e solution of low purityby the si ple eans of ! fast crystallization ethod∀. After the enzy e solution was purified by acetonea oniu sulfate cooperation precipitation, a oniu sulfate was quickly added to the enzy e solution. Then lu py crystals with the size of about40 30 were obtained. The crystalswere confir ed to be the SHP crystal through enzy e activity test. The resultshows that though the purity of enzy e solution is nothigh, crystals can stillbe for edwhen the enzy e solution rapidly reaches to a degree of super saturation, which is different fro the traditional ethods of protein crystallization. Additionally, a novelpurification ethod ofa oniu sulfateacetone cooperation precipitationwas also studied. The new ethod can reduce the procedure and obtain a great efficiency. Key words: soybean hullperoxidase; crystallization; purification [7] ( Crosslinked Enzy e Crystals, 4000 CLECs ), [ 8 9] , pH [ 1 2] [ 10] G , , 1000