冻融的肺癌细胞对骨髓树突状细胞的免疫调节作用.pdfVIP

冻融的肺癌细胞对骨髓树突状细胞的免疫调节作用 煤炭总医院肿瘤微创治疗中心 王洪武 黄友章 whw@ 摘要 目的 观察体外培养的肺癌细胞(NCI－H446 细胞)经氩氦刀处理后，肺癌细胞本身的变化， 变化了的肺癌细胞能否有效地激发骨髓树突状细胞（DCs）产生特异性抗瘤效应。方法 取体 外培养的肺癌细胞经氩氦刀处理后，在体外 DCs 培养过程中，加或不加经冻融的肺癌细胞， 分别观察肺癌细胞的形态结构，混合淋巴细胞反应、细胞毒 T淋巴细胞杀癌细胞效应。结果： 骨髓 DCs 培养后，培养细胞符合 DCs 特征。在培养过程中加入冻融的肺癌细胞，不影响 DCs 细胞表面抗原的表达，混合淋巴细胞反应增强，细胞毒 T淋巴细胞可引起肺癌细胞凋亡。结 论 经氩氦刀处理的肺癌细胞，细胞溶解，胞膜不完整，体外能有效地激发骨髓 DCs 产生特 异性抗瘤效应。 关键词 肺癌 冷冻 细胞抗原 树突状细胞 免疫 Immunomodulatory effects of cryolytic lung cancer cells on bone marrow-derived dendritic cells Wang Hongwu, Huang Youzhang Minimal Invasive Tumor Therapy Center, Beijing Meitan General Hospital,Beijing, PR China Email:whw@ Abstract: Cryosurgery is an emerging treatment for human solid tumors. This study was aimed to evaluate whether cryolyted NCI－H446 lung cancer cells could activate bone marrow-derived dendritic cells (DCs) which stimulate lymphocytes to be cytotoxic lymphocytes(CTLs) to kill cancer cells. Cultured lung cancer cells were cryo-treated and added to cultured DCs in the presence of naive lung cancer cells. Then cancer cells were evaluated for their morphological changes. IL-12 secretion by DCs, MLR and antitumor activity of CTLs were also evaluated. Results showed that lung cancer cells lysis and cell membrane collapse were obvious after cryo-treatment. The phenotypes of bone marrow-derived DCs were not affected by addition of cryolytic cancer cells, while IL-12 secretion, MRL and antitumor activity of CTLs were significantly enhanced. Thus cryo-treatment of lung cancer cells could result in cell apoptosis and be helpful for the cancer cells to activate bone marrow-derived DCs, which can make CTLs