磷脂跨膜交换机制研究.docVIP

磷脂跨膜交换机制的研究( 薛永培1, 梁化兰1, 尼亚孜别克1, 蒋中英1,2? (1. 伊犁师范学院电子与信息工程学院, 伊宁, 835000 2. 新疆大学物理科学与技术学院, 乌鲁木齐, 830046) 摘 要：膜间磷脂交换是一项重要的生理活动, 其对药物运输及膜功能研究有重要意义. 本文用石英晶体微天平及耗散系数测试仪研究囊泡与囊泡、囊泡与支撑膜间磷脂交换行为, 荧光光谱仪用来测量膜表面电性与膜组分对磷脂交换的影响. 实验结果表明: 磷脂跨膜交换速率与交换时间成反比, 膜表面异电性磷脂的增加会加速膜内相互作用和磷脂跨膜交换速率, 以及改变膜表面组分会对囊泡与支撑膜间的磷脂交换产生影响. 本文研究有助于加深理解磷脂跨膜交换机制, 并对药学研究提供参考. 关键词：石英晶体微天平, 支撑膜, 带电磷脂, 交换速率 中图分类号: 0485 文献标识码: A 文章编号: The Research of phospholipids exchange mechanism across membrane * XUE Yong-Pei1, LIANG Hua-Lan 1, NI Ya-Zi-Bie-Ke1，JIANG Zhong-Ying1,2? (1. School of Electronic and Information Engineering, Yili Normal University, Yining 835000, China; 2. School of Physics Science and Technology, Xinjiang University, Urumqi 830046, China) Abstract: Inter-membrane lipid exchange is an important physiological activity, which is important in drug delivery and the study of function of membrane. In this paper, quartz crystal microbalance with dissipation monitor(QCM-D) was applied to study the lipid exchange of vesicles-vesicles, small unilamellar vesicles(SUVs) and the supported lipid bilayers(SLBs), and fluorescence spectrometer was used to moniter the effects of membrane surface charge and membrane composition on the lipid exchange. It was found that lipids exchange time is inversely proportional to the inter-membrane lipid exchange rate, the addition of oppositely charged lipids in membranes improves the inter-membrane interaction and lipid exchange, and the changes of membrane composition have effect on the inter-membrane lipid exchange. The study may be helpful to understanding the mechanism of lipid exchange deeply and it also provides guidelines about the pharmaceutical research. Keywords: Quartz crystal microbalance, supported lipid bilayers, charged phospholipids, exchange rate 1 引 言 细胞是一切生物体的基本组成单元, 而细胞膜在其中起着重要的作用, 它与许多生命现象和生命活动都息息相关, 是能量转换、物质运输、生物传感和信号传导得以进行的重要场所. 然而由于细胞膜的结构较为复杂, 实验通常建立结构简单的模型用来研究其结构, 这些模型包括小单层囊泡(SUVs), 大单层囊泡(GUVs)[1] 支持磷脂双层膜等. 生物膜中、不同的磷脂组分直