Analysis of Methylation-Sensitive Amplified Polymorphism and Prediction of Candidate Genes Infected by SCMV in Zea mays Genome.pdfVIP

Analysis of Methylation-Sensitive Amplified Polymorphism and Prediction of Candidate Genes Infected by SCMV in Zea mays Genome.pdf

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M ar.2013,Vo1.7,No.3,PP.227-235 JournalofLi~ Sciences,ISSN 1934—7391,USA AnalysisofMethylation-S·ensitiveAmplified Polymorphism andPredictionofCandidate GenesInfectedbySCMV in LiLiu,,Xiu—JingHe,Zhi.M ingZhang,Mao.JunZhao。andGuang—TangPan PersonnelDepartment,SichuanAgriculturalUniversity,Yaan625014,China 2MaizeResearchInstitute.SichuanAgriculturalUniversiyt~KeyLaboratoryofCropGeneticResourcesandImprovementofChinese MinistryofEducation,Chengdu611130,China 3DepartmentofLfieSciences,SiehuanAgriculturalUniversiyt,Ya’an625014,China Received:January28,2013/Accepted:March11,2013/Published:March30,2013 Abstract:DNA methylationisanimportantcomponentoftheepigeneticnetwork.anditplaysimportna trolesingeneexpression regulationandepigeneticchangeresponsetovraiousstresses.Inthisstudy.theauthorsassessedthemethylationpaRemsstressedbv SCMV (sugarcanemosaicvirus)inmaizebymethylation-sensitiveamplifiedpolymorphism (MSAP),nadidentifiedimportant cna didategenesrelatedtoSCMV resistnacethrough combiningmicroarrayanalysiswithCpG islandsprediction.Theresultsof M SAPindicatedDNA meth2~rlationlevelsappeareddynamicchangesinoculatedfor0d ld,4d,5dand10d.1l8cnadidategenes wereidentifiedinfectedby SCMV.whichmayparticipateinDNA methylationmodification.Amongthem,eightcandidategenes weremappedonScmvlandScmv2QTLregions.whicharecurcialofrSCMVresistance.Inconclusion,DNAmethylationisclosely relatedwithmaizeresistnacetoSCMVandplaysanimportantroleinregulatinggeneexpressionrespondedtomaizeresistance. Keywords:Maize,SCMV,DNA methylation,M SAP,CpG islands 1.Introduction methylation,to maintain this genomic plasticity, allowingrelativelyrapidadaptationtonew conditions Bioticand abiotic stressesare importantlimiting and regulating genome functionsto a large extent factorsforcropgrowthandimprovementsin yields.

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