碳源对_聚谷氨酸发酵的影响.pdfVIP

2010 No．3 · · Serial No．216 China Brewing 70 Research Report 碳源对γ-聚谷氨酸发酵的影响 缪 静，杨在东，冯志彬，张玉香，程显好 （鲁东大学生命科学学院，山东烟台 264025） 摘 要：以γ-聚谷氨酸生产菌yt102为供试菌株，研究了碳源对γ-聚谷氨酸发酵的影响。首先通过摇瓶实验确定发酵的最佳碳源为葡 萄糖和柠檬酸，二者按一定的比例混合更有利于聚谷氨酸的产生，进一步利用10L发酵罐补料分批发酵确定碳源的最佳用量为40g/L， 继续优化培养条件，确定采用溶氧控制的脉冲补料方式可有效延续γ-聚谷氨酸的合成。在最优发酵条件下，通过10L发酵罐补料分批 发酵50h，γ-聚谷氨酸产量可达34.5g/L。 关 键 词：γ-聚谷氨酸；碳源；枯草芽孢杆菌；优化培养 中图分类号：Q934 文献标识码：A 文章编号：0254-5071 （2010）03-0070-03 Effect of carbon source on γ-polyglutamic acid production MIAO Jing, YANG Zaidong, FENG Zhibin, ZHANG Yuxiang, CHENG Xianhao (College of Life Science, Ludong University, Yantai 264025, China) Abstract: Effect of carbon source on the production of γ-polyglutamic acid (γ-PGA) were studied with Bacillus subtilis yt102. Effects of different carbon sources on γ-polyglutamic acid yield and cell mass were evaluated with shaking flasks. The results showed that glucose and citric acid were the optimal carbon sources, and production of γ-polyglutamic acid was improved when using combined two carbon sources. Futher study showed that high γ-polyglutamic acid yield was obtained when 40g/L carbon source was added to fermentation medium and DO-control pulse fed-batch was adopted. Under optimum conditions, the production of γ-polyglutamic acid could reach 34.5g/L. Key words: γ-polyglutamic acid; carbon source; Bacillus subtilis; optimization of fermentation γ-聚谷氨酸（γ-PGA）是由D型或L型谷氨酸通过α-氨 发酵培养基：葡萄糖 30g/L ，谷氨酸钠30g/L ，CaCl2 基和γ-羧基间的γ-酰胺键连接而成的多肽分子，通常由5000 1g/L，KH PO 1.5g/L，MgSO 0.5g/L，酵母粉30g/L，柠檬酸