视黄酸在早期形觉剥夺性近视豚鼠后巩膜中的变化-眼科研究20年.pdfVIP

眼科研究 2009年 12月第 27卷第 12期 1115 · · 实 验 研 究 · 视黄酸在早期形觉剥夺性近视豚鼠后巩膜中的变化 赵 小云 黄 悦 赵 少贞 Changesofretinoicacid levelin guineapigscleraofearlyform deprivationmyopia ZhaoXiaoyun，HuangYue，ZhaoShaozhen．TianjinMedicalUniversityEyeCenter，Tianjin300070，China Abstract 0bjective Researchesshowedthatretinoicacidmaybeasignalmolecularintheregulationofocularaxial length．Sclera isatargettissueofthepathogenesisofform deprivationmyopia．Recently．guineapigs reused tothe studyofthe pathogenesisofform deprivationmyopia．However，seldom studiesarefound in thechangeofretinoicacid levelin thescleraof guineapigs．Thisstudywasdesignedtoinvestigatetheroleofretinoicacidofearlyofrm deprivationmyopiainguineapigssclera． M ethods Monocularform deprivation modelswereestablished in 24 3-week—old guineapigsby occluding the lefteyeswith facemaskandrandomlydivided intoform deprivation 15daysgroup and24 daysgroup．Other6normalguineapigswereusedas controlgroup．Thefellow eyeswereused as internalcontro1．Refraction wasmeasured before and afterexperimentby using retinoscopy．Eyeballswereextracted immediatelyatthecorrespondingtimepointsandocularaxiallengthwasmeasured with outsidemicrometer．Thelevelofretinoicacid in scleralsampleswasdetectedbyhighperformanceliquidchromatography．Theuse ofanimalfollowedtheRegulationsofrtheAdministration ofAffairsConcerningExperimentalAnimalsbytheStateScienceand TechnologyCommission． Results Refractivediptorreducedby 一3．14±1．50D in experimentaleyesafterofrm deprivation． showingstatisticallydifferencesamongbeforeandvarioustimepointsafterform deprivation(F=23．053，P=0．000)，andno significantdifferencewasseeninreduceofrefractivediptorinfelloweyesafterofrmdeprivation(一1．24±1．99D)(F=1．506， P=0．240)．Theaxiallengthwasconsiderablyincreasedinform