三羟异黄酮对豚鼠心室肌细胞L-型钙通道电流的影响.pdfVIP

466 Acta Physiologica Sinica, August 25, 2004, 56 (4): 466-470 Research Paper Effect of genistein on L-type calcium current in guinea pig ventricular myocytes JI En-Sheng, YIN Jing-Xiang, MA Hui-Jie, HE Rui-Rong* Department of Physiology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, China Abstract : This paper was aimed to study the effect of genistein (GST) on L-type calcium current (ICa,L) in isolated guinea pig ventricular myocytes using whole cell patch-clamp recording technique. The results are as follows. (1) GST (10, 50, 100 µmol/L) reduced the voltage- activated peak amplitude of ICa,L in a concentration-dependent manner. Daidzein (100 µmol/L), a structural analogue of GST which has little or no inhibitory effect on tyrosine kinase, produced no effect over the same concentration range on ICa,L (n=5, P0.05). (2) GST up- shifted the current-voltage (I-V) curve, but the characteristics of I-V relationship were not significantly altered, and the maximal activation voltage of ICa,L was not different from that of control. GST did not affect the activation kinetics of ICa,L . (3) GST markedly shifted the steady-state inactivation curve of I to the left, and accelerated the voltage-dependent steady-state inactivation of I . V value was –28.6 ± 0.6 mV in Ca,L Ca,L 0.5 the control and –32.8 ± 1.1 mV in the presence of GST. The κ values were 5.8 ± 0.5 mV and 6.5 ± 0.9 mV, respectively (n=6, P0.05). (4) GST markedly shifted the curve of time-dependent recovery of Ica,L from the steady-state inactivation to the right, and slowed down the recovery of Ica,L from inactivation (n=7, P0.0