CT灌注成像评价骨髓间充质干细胞移植促进兔长期缺血后肢血管新生的实验的研究.pdfVIP

目 录 一、摘要 中文论著摘要··················································1 英文论著摘要··················································3 二、英文缩略语····················································5 三、论文 ． 前言··························································6日U舌。。。。。’。。。。。。。。‘‘。‘··‘·‘。·································。6 材料与方法····················································7 实验结果······················-·······························10 讨论··························································12 结论··························································14 四、本研究创新性的自我评价········································15 五、参考文献······················································16 六、附录 综述··························································19 致谢··························································27 个人简介······················································28 ·中文论著摘要· CT灌注成像评价骨髓间充质干细胞移植促进兔长期缺 血后肢血管新生的实验研究 目 的 探索研究骨髓间充质干细胞移植在兔长期缺血后肢血管再生中的作用及应用 CT灌注成像评价其毛细血管床的变化。 方 法 后肢股动脉离断造成后肢缺血，按慢性实验动物饲养60天，制成左后肢长期缺血 模型。于术后第6l天，将骨髓问充质干细胞悬液分别注射于实验组兔左后肢缺血 模型的股动脉周围的大腿肌肉群内，对照组兔左后肢注射同等剂量生理盐水。于 One 640层CT)灌注扫描，在 干细胞移植术后第28天进行CT(东芝Aquilion PerfusionCT软件进行灌注图像处理。于CT灌注扫 AquilionOne工作站用Body 描后当同处死兔，分别切取双侧内收肌进行组织形态学观察及免疫组化测定。主 要观察指标：两组兔左后肢与右后肢肌肉组织的血容量(Bv)、血流量(BF)的 microvessel 比值和内收肌毛细血管密度比值(radio density，rMVD)。 结 果 移植组血管生成情况明显优于未移植组。 结论 1．骨髓间充质干细胞具有促进长期缺血兔后肢血管生成的作用。 2．640层CT灌注成像可以无侵袭性、半定量评估兔后肢毛细血管床的变化。 关键词 骨髓间充质干细胞；移植；血管生成；体层摄影术；X线计算机；灌注；毛 细血管 2 ·英文论著摘要·