肿瘤坏死因子和干扰素对肠上皮紧密连接的破坏作用.pdfVIP

33 13 6 Vo.l 13 No. 6 2006 11 Parenteral Enteral Nutrition Nov. 2006 张 强, 李秋荣, 王 萌, 马 健, 黎介寿 ( , 210002) : 目的: (TNF-) ( IFN-), TNF- IFN- 方法: T 84 % DMEM /F-12 : TNF-IFN-TNF- IFN-TNF- IFN- 10 ng/mL TNF- 100 U /mL IFN- 72 h 100 U /mL IFN- 19 h, 10 ng/mL TNF- EVOM (TER ), 结果: IFN- 48 h , TER 78. 06% , 72 h 9. 31% TNF-, 24 hTER , 72 h 6. 82% , TNF- IFN- T84 TER, 72 h TER 20. 88% , 结论: TNF- IFN- T 84, : ; ; ; * : Q73 : A : 1007-810X( 2006) 06-033 -03 Synergistic effects of interferon-and tumour necrosis factor-on intestinal epithelium tight junction ZHANG Q iang, L IQ iu-rong, WANG M eng, MA Jian, LI Jie-shou (Research Institute of General Surgery, Nanj ing GeneralH osp ital of N anj ing M iliterey Command, PL , N anj ing 210002, Jiangsu, China) Abstracts: O bj ective: Inflamm atory bow el disease ( IBD) is characterized by chron ic intestinal inflam- mation and increased ep ithelial permeability. Both tumour necrosis factor-(TNF-) and interferon- ( IFN-) have been mi plicated in IBD. To understand better d isruption of ep ithelial barrier function by these cytokines, w e investigated the effects of IFN-andTNF-on intestinal epithelium tight junction. M ethods: T84 cellsw ere grow n in a 1 1 m ixture of DMEM and F-12 medium supplemented w ith % heat-inactivated fetalbovine serum. The cellmonolayersw ere incubated w ith IFN-( 100 U /mL), TNF- ( 10 ng/mL) or the comb inat