生物表面活性剂产生菌的筛选及表面活性剂稳定性研究_牛明芬.pdfVIP

Ch nese J ournal of Ecology 2005, 24( 6) : 63 1~ 634 * 1, 2 2 1 2* * 2 2 2 牛明芬 李凤梅 韩晓日 郭书海 牛之欣 冷延慧 张春桂 ( 1 沈阳农业大学土地与环境学院, 沈阳 110 16 1; 2 中国科学院沈阳应用生态研究所, 沈阳 110016) , , 52 , B22 B24 B25, pH N aCl , B22 120 , 2 80 ; 3 pH , B22 pH 40 ~ 130 , B24 B25pH 20~ 130; Na Cl 3 , 72 h 70 % , , Q935 A 1000- 4890 ( 2005) 06- 0631- 04 Isolation of biosurfactantproducing microorganisms and their stability. N IU M ngf en1, 2 , L I Feng me 2, HAN 1 2 2 2 2 1 X aor , G UO Shuha , N IU Zh x n , L EN G Yanhu , ZHAN G Chungu ( ( College of L and and Env iron 2 ment, S heny ang A gricultural University, Shenyang 1100 16, China; I nstitute of Applied Ecology, Chinese A cademy of Sciences, Shenyang 110016, China) . Chinese ournal of Ecology , 2005, 24( 6) : 631~ 634. 52 b osurf actantproduc ng m croo rgan sms w ere solated w th the methods of enr chment culture and plate cult vat on fro m o l sludge of Daq ng O l F eld . Exper ments of o l d splacement act v ty and surf ace tens on show ed that the three stra ns ( nclud ng B22 , B24 and B25 ) had h gh surf ace act v ty and lo w surf ace tens on. T he analys s of phys cochem cal propert es nd cated that the b osurf actant produced by B22 could bear h gh temperatur e up to 120 , w h le the ot her tw o could bear 80 . T he three b osur factants had a w de adapt ab l ty to pH . B22 had a w de adaptable pH rang e of 4 . 0 ~ 13 . 0, w h le that o f B24 and B25 w as 2 . 0~ 13. 0. Na Cl co ncentrat