骨舒汤对膝骨性关节炎家兔模型形态学观察及bFGF表达的影响.pdf

中文摘要 目的 本课题旨在中医理论指导下,结合现代科学的研究方法,通过对兔膝 骨性关节炎的实验及临床研究,观察复方中药骨舒汤对膝骨性关节炎家兔模型形 态学观察及bFGF表达的影响,探讨其治疗KOA的疗效机制,并为进一步临床运用及 推广奠定基础。 方法 将48 只健康成年日本大耳白兔随机分为4 组:A空白组、B 模型组、C 壮骨关节丸组、D 骨舒汤组, 每组12 只，各称重标记。除空白组外均采用Hulth [1] 法 造模，切断前交叉韧带,注意勿损伤关节软骨。造模成功后，A 组和B 组均采 用蒸馏水 10ml 灌胃,不加其他成分；C 组给予壮骨关节丸 10ml(0.7g/ml)水溶液 灌胃,D 组给予骨舒汤10ml(0.7g/ml)水溶液灌胃。连续给药8 周后，处死各组兔 子，取滑膜、关节软骨标本行HE 染色光镜形态学观察[2]及软骨病理评分，用免疫 组织化学方法检测关节软骨中bFGF 的表达。 结果 骨舒汤可以明显上调关节软骨中bFGF的表达。实验结果显示模型组发 生明显的膝关节滑膜炎性改变及软骨退行性改变；C组、D组病理积分明显优于模 型组(P0.01)；C组、D组病理积分比较有显著性差异。术后各期灰度值均显著低 于模型组(P0.01)，表明C组、D组可显著上调bFGF表达。肉眼与光镜观察C组、D 组滑模和关节软骨的病理改变明显轻于模型组。 结论 骨舒汤可能是通过上调关节软骨中bFGF的表达,改善病理积分，从而 抑制炎症介质的释放，减轻滑膜炎症，改善软骨细胞的功能，延缓关节软骨退变， 保护关节软骨的作用，从而对于骨性关节炎起到一定的治疗作用。 关键词 骨舒汤； 骨性关节炎； 软骨； bFGF； 实验研究 Gushu decoction on knee osteoarthritis rabbit model of morphology and its effect on bFGF expression Experimental Study Abstract Objective: This topic seeks to under the guidance of TCM theory, combined with modern scientific research methods, through the rabbit knee osteoarthritis experimental and clinical research, observation of compound Chinese medicine Gushu decoction on knee osteoarthritis rabbit model of morphology and its effect on bFGF expression, and to explore the mechanism of therapeutic efficacy of KOA, and to further lay the foundation for clinical application and promotion. Methods: 48 healthy adult Japanese white rabbits were randomly divided into four groups: A Blank group, B model group, C bolus group, D Gushu decoction groups of 12 rats, each weighing mark. In addition to the blank group are used outside the Hulth method [1] modeling, cut off anterior cruciate ligament, pay attention not to damage articular cartilage. Modeling is successful, A group and B group were fed with 10ml of distilled water, without other ingredients; C group were given bolus 10ml (0.7g/ml) aqueous gavage, D