RF-PECVD聚合类聚乙烯氧(PEO-like)薄膜的蛋白质吸附性研究.pdfVIP

RF-PECVD聚合类聚乙烯氧（PEO-like）薄膜的蛋白质吸附性研究 1 1 1 1 2 胡文娟 ，谢芬艳 ，付亚波 ，陈强 *,翁静 1-北京印刷学院印刷包装材料与技术北京市重点实验室，北京大兴，102600； 2-首都医科大学基础医学部，北京 100069 北京印刷学院等离子体物理及材料研究室，北京大兴，102600 摘要：本文采用射频等离子体（13.56MH）,以乙二醇二甲基醚（EthyleneGlycolDiMethyl Ether）为聚合单体,氩气为辅助气体,合成类聚乙烯氧（PEO-like）功能聚合薄膜。采用连续与 脉冲射频（RF）等离子体两种放电模式聚合PEO功能薄膜。实验研究了等离子体放电参数：如等离 子体放电功率、工作气压、放电模式（连续或脉冲）、聚合时间等对聚合物表面结构、功能团含量 表面成分以及性能和蛋白质吸附等的影响。利用接触角测定仪（WCA）、傅立叶变换红外光谱(FTIR)、 原子力显微镜（AFM）等手段对其进行结构、成分和形貌分析。同时本文也采用了体外细胞培养的 方法研究了类PEO功能薄膜对富血血小板以及蛋白细胞的吸附。通过倒置显微镜观察细胞黏附的数 量和形态。结论是：采用RF-PECVD可以在连续等离子体模式下较小的功率，或脉冲等离子体模式 下较长时间得到结构、性能优异的PEO生物功能薄膜，所制备的PEO生物功能薄膜具有良好的血液 相容性以及抗蛋白吸附性能。 关键词：RF-PECVD；PEO-like；血液相容性； 蛋白吸附 PEO-like film polymerization by RF-PECVD and its adsorption of protein Abstract ：The paper presents the PEO -like functional film was polymerized by plasma-enhanced vapor chemical deposition (PECVD) on P-Si(100) surface with precursor EGDME and diluted Ar in continuous wave (CW) and pulse plasma mode. We explored the effect of plasma parameter such as the discharge power, working pressure, the discharge mode on the film properties. The structure of coatings was analysed by Fourier transform infrared (FTIR) spectroscopy, contact angle measurement and Atomic Force Microscope (AFM). And we obtain that the lower power in CW or the longer Toff in puls ed plasma is contribute to more density of EO on the surface. At the same time, this paper also studied in vitro the adsorption of platelet -rich plasma (PRP) and protein cells , and observed the configuration of attached cell by invert microscope. We concluded that the plasma polymeriz ation PEO -like functional film with outstanding structure and performance, can either in the smaller power CW, or in the longer Toff pulsed plasma mode, in w