百草枯与牛血清白蛋白结合作用荧光光谱.pdfVIP

2005 63 Vol. 63, 2005 18 , ACTA CHIMICA SINICA No. 18, · · *,a a b a a a (a 434020) (b 430072) , , (PQ) (BSA). , PQ BSA . Stern-Volmer Lineweaver-Burk , BSA PQ , , . Förster 212 2.07 nm. Lineweaver-Burk K 4 1 4 1 LB (297 K: 2.035 10 L•mol ; 304 K: 3.256 10 L•mo ; 311 K: 2.889 4 1 1 1 1 1 10 L•mol )( ∆H 18.50 kJ•mol ; ∆S 144.7 J•K /145.2 J•K /141.0 J•K ; ∆G 24.50 kJ•mol1/ 25.66 kJ•mol1/ 25.36 kJ•mol1), . PQ BSA , PQ . ; ; ; ; ; Fluorescence Spectra of the Binding Reaction between Paraquat and Bovine Serum Albumin YAN, Cheng-Nong*,a ZHANG, Hua-Xina LIU, Yib MEI, Pinga LI, Ke-Huaa TONG, Jin-Qianga (a College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou 434020) (b College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072) Abstract Under the imitated physiological condition of animal body, the binding of paraquat to bovine serum albumin (BSA) was studied by fluorescence spectrum and ultra-violet spectrum. It was shown that this compound has a quite strong ability to quench the