败酱草高速逆流色谱法分离纯化化合物并考察了化合物体外抗肿瘤活性.pdfVIP

Journal of Chromatography A, 1115 (2006) 103–111 Preparative isolation of four new and two known flavonoids from the leaf of Patrinia villosa Juss. by counter-current chromatography and evaluation of their anticancer activities in vitro Jinyong Peng, Guorong Fan ∗, Yutian Wu Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, China Received 9 December 2005; received in revised form 23 February 2006; accepted 24 February 2006 Available online 20 March 2006 Abstract A preparative counter-current chromatography (CCC) was used to isolate and separate chemical constituents from the leaf of Patrinia villosa, a famous traditional Chinese medicinal herb. Six flavonoids including two known and four novel compounds were successfully simultaneous purified by CCC with a two-phase solvent system composed of n-hexane–ethyl acetate–methanol–water (10: 13: 13: 10, v/v) by increasing the flow rate of the mobile phase from 1.0 ml/min to 2.0 ml/min after 110 min to bring out the late eluters. The separation produced total of 44.9 mg fraction I with 99.1% purity, 35.5 mg fraction II with 98.8% purity, 79.8 mg fraction III with 99.3% purity, 45.8 mg fraction IV with 98.8% purity, 39.8 mg fraction V with 98.6% purity and 9.6 mg fraction VI with 97.5% purity from 400 mg crude extract in one sin- gle isolation procedure and less than 10 h, and the obtained fractions were all analyzed by high performance liquid chromatography (HPLC). Their chemical structures were elucidated as (2S)-5,7