壳聚糖纳米ZrO2多壁碳纳米管纳米复合膜固定葡萄糖氧化酶构建新电流型葡萄糖生物传感器.pdfVIP

New amperometric glucose biosensor by entrapping glucose oxidase into chitosan/nanoporous ZrO2/multiwalled carbon nanotubes nanocomposite film Xiurong Zhai ， Jinxiang Zeng ，Yanping Gao, Shuguo Gong ，Wanzhi Wei ∗ (State key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China) Abstract A new nanocomposite material for construction of glucose biosensor was proposed. The biosensor was formed by entrapping glucose oxidase in chitosan (CHIT)/nanoporousZrO2/multiwalled carbon nanotubes (MWNTs) nanocomposite. In this biosensing thin film, MWNTs can effectively catalyze hydrogen peroxide and nanoporous ZrO2 can enhance the stability of the immobilized enzyme. The resulting biosensor provides a very effective matrix for the immobilization of Gox and exhibites a wide linear response range of 8 -1 -1 µmol·L to 3 mmol·L with a correlation coefficient of 0.994 for the detection of glucose. And the response -1 time and detection limit (S/N=3) of the biosensor are determined to be 6 s and 3.5 µmol·L , respectively. Another attractive characteristic is that thebiosensor is inexpensive, stable and reliable. Keywords: nanocomposite, glucose oxidase, nanoporous ZrO2, multiwalled carbon nanotubes, chitosan, biosensors were continuously being developed. However, some techniques were somewhat Introduction complex and the enzyme cou