纤维定向排列和纤维机械性能之间关系.pdfVIP

ARTICLE IN PRESS Biomaterials 27 (2006) 3136–3146 /locate/biomaterials Characterisation of electrospun polystyrene scaffolds for three-dimensional in vitro biological studies a b,c b b d Simon C. Baker , Neil Atkin , Paul A. Gunning , Nick Granville , Karen Wilson , Darren Wilsonb, Jennifer Southgatea, aJack Birch Unit of Molecular Carcinogenesis, Department of Biology, University of York, Heslington, York, YO10 5YW, UK bSmith and Nephew Research Centre, York Science Park, Heslington, York YO10 5DF, UK c (Current Address) Reckitt Benckiser Healthcare, Dansom Lane, Hull HU8 7DS, UK dDepartment of Chemistry, University of York, Heslington, York YO10 5YW, UK Received 27 September 2005; accepted 24 January 2006 Abstract The purpose of this study was to produce a well-characterised electrospun polystyrene scaffold which could be used routinely for three- dimensional (3D) cell culture experimentation. A linear relationship (p o0:01) between three principal process variables (applied voltage, working distance and polymer concentration) and fibre diameter was reliably established enabling a mathematical model to be developed to standardise the electrospinning process. Surface chemistry and bulk architecture were manipulated to increase wetting and handling characteristics, respectively. X-ray photoelectron spectroscopy (XP