自体骨髓干细胞移植治疗肝衰竭与初步观察.docVIP

自体骨髓干细胞移植治疗肝衰竭的初步观察 Preliminary effect of autologous bone marrow~derived stem cell transplantation for liver failure 巫贵成1 王 辉1 钟 庆1 何泽清2 邓元明3 WU Guicheng1 WANG Hui1 ZHONG qing1 HE Zeqing2 DENGYuanming3 1 重庆三峡中心医院肝病中心（中国 404000） 2 重庆三峡中心医院CT、MRI科（中国 404000） 3重庆三峡中心医院放射科（中国 404000） （1.Department of Liver Disease，2. Departmenr of Radiology，3.Department of CT、MRI, Chongqing Three Gorges Central Hospital, Chongqing ,404000,China 中图分类号：R575.3 文献标识码：A 文章编号：1818－0086（2011）06 摘 要：目的 观察自体骨髓干细胞移植治疗肝衰竭的初步疗效 方法12例经常规内科综合治疗无明显效果的肝衰竭患者。在无菌条件下采集骨髓100~200ml，通过密度梯度离心分离纯化出骨髓干细胞，通过介入的方法经肝动脉注射入肝内，静脉输注肝细胞生长素1~2周。观察移植后1月、2月、3月和6月患者症状、体征、肝脏生化和凝血酶原时间等的变化 结果 9例肝衰竭患者的症状、体征改善，白蛋白升高、胆红素降低，肝脏功能改善，所有患者未见明显副作用 结论 自体骨髓干细胞移植治疗肝衰竭安全有效 关键词：骨髓干细胞；移植；肝衰竭 Abstract：Objective To evaluate the preliminary effect of autologous bone marrow~derived stem cell transplantation for liver failure. Methods Twelve patients with liver failure were studied. 100~200 ml bone marrow from the patients was harvested in aseptic conditions. After isolation and purification by density gradient centrifugation, the stem cells were obtained and transplanted into the liver via hepatic artery. All patients were injected hepatic growth factor for 1~2 weeks. Clinical symptoms and signs and liver function and prothrombin time were followed up at the l month, 2month, 3month, 6month after transplantation. Result After transplantation, clinical symptoms and signs and laboratory data of 9 patients were improved and levels of albumin was increased insignificantly. Complication and serious side~effect were not observed. Conclusion The treatment for liver failure by autologous bone marrow~derived stem cell transplantation is safe and effective. Key Words： Bone marrow~derived stem cells;Transplantation;Liver failure 目前各种原因所致的肝衰竭仍然是临床医生面临的一大挑战。干细胞由于具有良好的增值和分化能力已被证明可以修复许多受损的组织而日益受到重视，近年来已有不少采用干细胞移植治疗传统疗法不佳的疾病的报道，其疗效令人鼓舞。此研究初察了经肝动脉自体骨髓干细胞移植治疗肝衰竭的近期疗效，现报道如下： 1对象与方法 1.1 对象 重庆三峡中心医院2009年1月~2010年3月就诊的肝衰竭患者12例，所有患者符合肝衰竭诊疗指南