多发性骨髓瘤患者外周血单个核细胞c-Cbl 和Cbl-b 基因表达下降.docVIP

多发性骨髓瘤患者外周血单个核细胞c-Cbl和Cbl-b基因表达下降方苏1，李萡1*，牛宇哲1,2，陈少华1，杨力1，李扬秋1,2（暨南大学1. 血液病研究所; 2. 再生医学教育部重点实验室，广东 广州 510632） [摘要] 目的了解多发性骨髓瘤（MM）患者外周血单个核细胞中c-Cbl和Cbl-b基因表达水平。方法利用SYBR Green 实时定量PCR方法，检测16例MM患者和12例健康人外周血单个核细胞c-Cbl和Cbl-b基因的表达情况，以β2微球蛋白基因(β2M)作为内参照，采用2-ΔCt×100%计算c-Cbl和Cbl-b基因相对表达水平。结果MM患者中c-Cbl和Cbl-b基因表达水平与健康人相比均下降，其中MM组c-Cbl(平均秩：12.56) 表达水平与健康对照组（平均秩：17.08）比较无统计学意义（p=0.159）；而Cbl-b（平均秩：10.72）表达水平与健康对照组（平均秩：19.54）则显著降低（p＜0.05﹚。结论本研究首次报道 MM患者中c-Cbl和Cbl-b表达水平的改变， c-Cbl和Cbl-b表达下调可能通过影响BCR和TCR信号传导通路从而影响机体的免疫调节功能。 [关键词] 多发性骨髓瘤（MM）；c-Cbl；Cbl-b；荧光定量PCR Decreased c-Cbl and Cbl-b genes expression in peripheral blood mononuclear cells from multiple myeloma patients Fang Su1,Li Bo1*,Niu Yuzhe1,2 ,Chen Shaohua1,Yang Lijian1,Li Yangqiu1,2(Jinan University 1. Institute of Hematology; 2. Regenerative Medicine, Ministry of Education Key Laboratory,Guangzhou,Guangdong province, 510632,China) ［Abstract］ObjectiveTo investigate the expression level of c-Cbl and Cbl-b genes in peripheral blood mononuclear cells of patients with multiple myeloma（MM）. MethodsThe SYBR Green quantitative real-time PCR was used for detecting c-Cbl and Cbl-b genes expression level in peripheral blood mononuclear cells（PBMC）of 16 patients with MM and 12 healthy individuals, β2-microglobulin gene (β2M) was used as an endogenous reference. Relative changes of c-Cbl and Cbl-b genes expression level were used by the 2-ΔCt×100% method. Results Decreased c-Cbl and Cbl-b genes expression level were found in MM patients, there no significant difference of c-Cbl gene expression level between MM patients (average rank：12.56) and healthy control（average rank：17.08）（p=0.159）. However, the expression level of Cbl-b gene in MM（average rank：10.72）was significantly lower than that in healthy control（average rank：19.54）（p＜0.05﹚. ConclusionIn this study, we firstly reported the change of c-Cbl and Cbl-b genes expression level in MM patients. The down-regulated expression level of c-Cbl and Cbl-b genes may lead to abnormality of B