体外诱导小鼠胚胎干细胞分化为肝细胞实验的研究.pdfVIP

Study on the Differentiation of Mouse Embryonic Germ Cells into hepatocytes Feng Zhihui, Liu Xiaoping, Guo Feifei Department of Histology and Embryology, Qingdao University Medical College, Qingdao （266021 ） Abstract Objective To investigate the condition which can induce embryonic germ cells (EGCs) to differentiate into hepatocytes in vitro, and lay bases for the study of mechanism of EGCs differentiation, so as to seek new source of seed cells for tissue engineering . Methods The EGCs deriving from gonadal ridges of the mouse embryos on the 11th day were cultured and proliferated in vitro. An aliquot EGCs suspension was cultured in 6-well dishes for 24 hours and then bFGF ,EGF, β-NGF, RA and hepatocyte abstraction were added into the dishes respectively for directional differentiation, and another aliquot EGCs was co-cultured with embryonic hepatic cells for differentiation. Morphological differentiation and intake of indiocyanine green (ICG) by EGCs were investigated. The committed differentiation of EGCs into hepatocytes was confirmed by albumin (ALB) immunocytochemistry. Results After addition of β-NGF, hepatocyte abstraction or co-culture with embryonic hepatic cells, the EGCs were differentiated into hepatocyte-like cells which were star-like or oval in shape. These hepatocyte-like cells ingested ICG and expressed ALB immunoreactivity. They could produce more ALB positive cells than control group (p0.05), bFGF, EGF and RA had no such effects. Conclusion β-NGF, some unknown factors from hepa