miR_181b在前列腺组织中表达及对前列腺癌细胞PC_3生物学功能影响.pdfVIP

·2042 · ， miR － 181b PC － 3 何 龙 等 在前列腺组织中的表达及对前列腺癌细胞 生物学功能的影响 miR － 181b 在前列腺组织中的表达及对前列腺癌细胞PC － 3 生物学功 能的影响 1，2 2 2 3 2 2 ， ， ， ， ， 何 龙 邱 实 刘 龙 姚 辉 范连慧 李 昕 The expression of miR－ 181b in prostate cancer and effects of miR－ 181b on the biolog- ical behavior of prostate cancer PC － 3 cell line 1，2 2 2 3 2 2 HE Long ，QIU Shi ，LIU Long ，YAO Hui ，FAN Lianhui ，LI Xin 1 2 Postgraduate Dep artment ，Liaoning Medical University ，Liaoning Jinzhou 121001，China ;Dep artment of Urology ，Shenyang Military 3 General Hosp ital ，Liaoning Shenyang 110016 ，China ;Dep artment of Digestive System ，Shenyang Military General Hosp ital ，Liaoning She- nyang 110016 ，China． 【Abstract 】 Objective :To study the expression of miR － 181b in specimens of prostate cancer and to investigate the effects of miR － 181b on the biological behavior of prostate cancer PC － 3 cell line． Methods :issues from 27 cases of prostate cancer and 30 samples of normal human prostate were collected after surgical operation． Total miRNA was ex- tracted and the relative expression of miR － 181b was quantified by Real － time PCR． miR － 181b ASO was transfect- ed into prostate cancer PC － 3 cell． The expressions of miR － 181b in transfected and non － transfected cells were measured by Real － time PCR ;The changes of cell apoptosis were measured by flow cytometry ;MTT assay and growth curve were used to assess the effect of miR － 181b on cell proliferation ;The changs of cell inva