甲醛降解菌分离纯化及其降解效果的研究论文 精简版.docVIP

小论文 甲醛降解菌的分离纯化及其降解效果研究 摘要：本文以甲醛为唯一碳源，从工业废水中分离得到2株甲醛降解菌，经形态学观察、生理生化特性研究，初步判定一株为假单胞菌，另一株为霉菌。通过单因素试验，分析两者的单菌株以及混合菌株甲醛降解效果：菌①的最适pH=7.5，转速为120 rpm；菌②最适pH=6.0，转速为80 rpm；菌①+②混合菌最适为pH=6.5，转速为100 rpm。在两因素三水平实验中通过在30 ℃培养24 h，找到pH和摇床转速对甲醛降解的共同影响：菌①为pH=8.0、转速 80 rpm，其甲醛降解率达到83.27%；菌②为pH=6.0、转速80 rpm，其甲醛降解率达到79.65%；菌①+②双菌混合培养时为pH=6.0、转速100 rpm，其甲醛降解率达到73.68%。 在常温情况下，分别保证两菌在最适条件下进行降解实验，结果显示在72小时时其甲醛降解率分别达到71.45%、 76.95%、72.90%。 关键词：甲醛；甲醛降解菌；筛选；降解特性；降解曲线 The separation and purification of formaldehyde degrading bacteria and their degradation effect research In this paper, using formaldehyde as a sole carbon source, from industrial waste water of 2 strains isolated from formaldehyde degradation bacteria, by morphological observation, physiological and biochemical characteristics of the strain, the preliminary determination for Pseudomonas, another one for mold. Through single factor analysis of both single-strain and hybrid strains of formaldehyde degradation effect: bacteria ① optimum pH = 7.5, the rotating speed is 120rpm; bacteria ② optimum pH = 6.0, the rotating speed is 80rpm; bacteria ① + ② mixed bacteria most suitable for the pH = 6.5, speed is 100rpm. In two experimental factors and three levels by 30 ℃ for 24 h, find the pH and shaking speed on the combined effect of the degradation of formaldehyde: for bacteria ① the pH = 8.0, speed 80 rpm, the formaldehyde degradation rate reaches 83.27%; bacteria ②,pH = 6.0, speed 80 rpm, the formaldehyde degradation rate reaches 79.65%; bacteria ① + ② pairs mixed culture of bacteria when pH = 6.0, speed 100rpm, the formaldehyde degradation rate is 73.68%. Two bacteria at normal temperature, respectively, to ensure optimal conditions for degradation experiments, the results showed that the formaldehyde degradation rate at 72 hours, respectively, 88.93%, 90.73%, 88.31%. Key words：Formaldehyde; Formaldehyde degradation strain; creening;degradation characteristics; degradation curve 引言 甲醛(HCHO)，又名“蚁醛”，在常温下是一种无色、有强烈刺激性气味的气体，易溶于水、醇醚，它有凝固蛋白质的