SOCS3在妊娠期肝内胆汁淤积症患者胎盘组织中表达及意义论文终稿.docVIP

SOCS3在妊娠期肝内胆汁淤积症患者胎盘组织中的表达及意义 张丽娟 李明月 ＊ (中南大学湘雅二医院妇产科 湖南长沙 410011) 【摘要】探讨细胞因子信号导因子3（uppressor of cytokine signaling3,SOCS3）在妊娠期肝内胆汁淤积症的表达及意义。采用免疫组化法44例ICP(其中轻23例，重21例)25例妇（对照组）的胎盘组织中SOCS3的定位与表达水平(阳性细胞率及平均灰度值表示)，采用ELISA的方法检测胎盘组织匀浆IL-10及I-γ的（）SOCS3主要滋养细胞表达SOCS3表达阳性细胞率平均灰度值ICP轻组ICP重组±0.12）、(81.06±6.79)]、[（0.42± 0.09）、(147.29±7.11)]、[(0.15±0.08）、(203.85±6.58)]，对照组、ICP轻型组、ICP重型组胎盘组织中SOCS3表达阳性细胞率依次降低，平均灰度值依次升高，差异有统计学意义（P0.001）。（3）IL-10及IFN-γ水平ICP轻组ICP重组1.39±0.08)ug/L、(10.52±0.32)ug/L]、[(1.22±0.75) ug/L、(13.41±0.47)ug/L]、[(1.16±0.68)ug/L、(16.84±0.69)ug/L]；IFN-γ/IL-10比值分别为(8.56±0.14)、(11.65±0.19)、(16.02± 2.79)， 对照组、ICP轻型组、ICP重型组胎盘组织匀浆中IL-10水平依次降低，而IFN-γ水平及IFN-γ/IL-10比值依次升高，差异有统计学意义（P0.001）。（）ICPICP重型组胎盘组织中SOCS3表达阳性细胞率与胎盘组织匀浆中IFN-γ/IL-10比值(P0.001)，而平均灰度值与IFN-γ/IL-10比值(P0.001)。 结论　（）SOCS3SOCS3可能参与ICP的发病，而且可能与病情进展有关。（）SOCS3表达与胎盘组织匀浆中IFN-γ/IL-10比值呈负相关，说明SOCS3可能在ICP的Th1/Th2细胞因子免疫调节中起了一定作用。 【】γ；IL-10 Expression and significance of socs3 in placenta of Intrahepatic cholestasis of pregnancy.ZHANG Li-juan,LI Ming-yue Department of Obstetrics and Gynecolo gy,Second Xiangya Hospital of Central South University,Chang sha 410011, China Corresponding author:Zhang-Li-Juan,Email:zlj621116@. 【Abstract】To investigate the effect of SOCS3 in the Pathogenesis of intrahepatic cholestasis of pregnancy (ICP).Methods The samples were obtained from 44 cases of ICP patients (ICP)including mild group(MG) (n=23)and severe group(SG) (n=21),25 cases of normal pregnancy women(NG).The expression and localization of SOCS3 in placentus were examined by immunohistochemical method. The level of IL-10 and IFN-γ from placenta homogenation were measured by ELISA. Results (1)The immunoreactivity of SOCS3 was mainly found in the intracytoplasm of trophocyte.(2) The rate of positive cells and average gray scale of SOCS3 in placentus in NG、in MG and in SG were [(0.69±0.12)、(81.06±6.79)] 、 [(0.42±0.09) 、(147.29± 7.11)] 、[(0.15±0.08）、( 203.85± 6.58)], respectively. In NG、in MG and in SG the rate of positive ce