热激胁迫可能通过降低脂肪与糖原的积累抑制涡虫的发育（英文）.pdfVIP

东 南大 学学 ． iv ．． JSoutheastUn 嚣(Med医‘Sc学iE版di)；20l0，Feb．29(1 41 36． 模式动物研 究特别报道 · Heat-shockstresssuppressesthedevelopmentpossiblybydecreasing lipidand glycogen storageinplanarianDugesia LIU Peidang，LINXing。feng，WANG Da。yong (KeyLaboratoryofDevelopmentalGenesandHumanDiseaseinMinistryofEducation，DepartmentofBiochemistry andMolecularBiology，SoutheastUniversityMedicalSchool，Ⅳn ng210009，China) A『bstract1Objective：Toinvestigatetheeffectsofheat—shockondevelopmentandaccumulationoflipidand glyc0geninplanarianDugesiajaponica．Methods：Thedevelopmentofplanarianswasevaluatedbythebodylength andbodvweight．Thebodylength，bodyweight，andtheaccumulationoflipidandglycogeninparenchymalcells wereexaminedinheat—sh0ck(35℃)treatedplanarians．Thepossibleassociationsofdevelopmentwithlipidor glycogenstoragewerefurtheranalyzedin heat—shocktreatedplanarians．Results：Afterheats‘hock treatmentfor difrenttimeinterva1s(16，20，24，28，and32h)，thebodyweightsandbodylengthsofheats‘hocktreated Dlanarianswereallsignificantlydecreasedcomparedwiththoseincontrolplanarianswithoutheat—shocktreatment． ComDaredwiththelipidaccamulationincontrolplanarians，heatshocktreatmentresultedinthesharpdecreaseof lipiddropletnumberandthereductionoflipiddropletsizeinparenchymalcells．Similarly，therelativeintensities oflabe1ed signalsfor glycogen in parenchymalcellswere significantly decreased afterheat。shock treatment· M0I_eover．1inearregressionanalysissuggeststhatboth thebodyweightand thebodylengthweresignificantly corre1atedwiththerelativesizeoflipiddropletandtherelativeintensityofglycogen signalinheat’shock treated Dlanarians．Conclusion：Treatmentwith the stressofheat—shock can obviously suppressthe developmentof plan ans，andthecloseassociationbetweenthedevelopmentandtheaccumulationoflipidorglycogenexistsin heat’shocktreated