用电渗析与超滤膜从雪蟹副产物水解液中分离肽中超滤膜材料影响.pdfVIP

ARTICLE pubs.acs.org/JAFC Impact of Ultrafiltration Membrane Material on Peptide Separation from a Snow Crab Byproduct Hydrolysate by Electrodialysis with Ultrafiltration Membranes Alain Doyen,†,‡ Lucie Beaulieu,†,§ Linda Saucier,†,# Yves Pouliot,†,‡ and Laurent Bazinet*,†,‡ †Institute of Nutraceuticals and Functionnal Foods (INAF) ‡Department of Food Science and Nutrition UniversiteLaval, Quebec City, QC, Canada, G1V 0A6 §Department of Biology, Chemistry and Geography, Universitedu Quebec a Rimouski (UQAR), Rimouski, QC, Canada G5L 3A1 #Department of Animal Science, UniversiteLaval, Quebec City, QC, Canada, G1V 0A6 ABSTRACT: Electrodialysis with ultrafiltration membrane (EDUF) is a technology based on the separation of molecules according to their charge and molecular mass. Some works have already successfully demonstrated the recovery of bioactive peptide fractions. However, the impact of ultrafiltration membrane (UFM) material, used in the EDUF system, on the peptide migration has never been studied. Consequently, the objectives of this work were (1) to evaluate the effect of two different UFM materials on the selective separation of peptides from a snow crab byproduct hydrolysate by electrodialysis with ultrafiltration membranes and (2) to determine the effect of UFM material on their potential fouling by peptides. It appeared that, after 6 h of EDUF separation using polyether sulfone (PES) and cellulose acetate (CA) UFM, peptides with low molecular weights ranging from 300 to 700 Da represented the most abundant population in the KCl1 (compartment located near the anode for the recovery of anionic/acid peptide fractions) and KCl2 (