稳定同位素技术分析浮游食物网的样品预处理方法研究-湖泊.pdfVIP

J. Lake Sci.(湖泊科学), 2009, 21(4): 465-473 . E-mail: jlakes@ ©2009 by Journal of Lake Sciences 湖泊底泥疏浚环境效应: Ⅲ .对沉积物反硝化作用的影响∗ 钟继承, 刘国锋, 范成新∗∗, 白秀玲, 李 宝, 张 路, 丁士明 ( 中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室, 南京 210008) 摘 要: 通过为期一年的疏浚模拟试验, 在试验室培养疏浚与对照柱样, 研究了底泥疏浚对沉积物反硝化过程的影响. 沉积 物反硝化速率的测定采用经典的乙炔抑制法, 研究结果表明, 在一年的试验周期内, 疏浚和对照柱沉积物的反硝化速率分别 为6.9-26.9nmol/(g⋅h)和21.6-102.7nmol/(g⋅h), 除2006 年的2 月外的其他月份, 疏浚沉积物的反硝化速率显著(P0.05)低于未 疏浚对照沉积物, 同时还研究了环境因子对沉积物反硝化速率的影响, 结果表明, 疏浚和对照沉积物的反硝化速率都受温度 的控制, 硝态氮浓度是疏浚和对照沉积物反硝化速率的主要限制因子, 有机碳对疏浚沉积物的反硝化速率有影响, 但对未疏 浚对照沉积物的反硝化速率没有影响. 疏浚后短期内沉积物反硝化速率低于未疏浚对照沉积物, 可看作是底泥疏浚在富营养 华水体脱氮方面的一个负效应. 关键词: 底泥疏浚; 反硝化; 环境因子; 太湖 Environmental effect of sediment dredging in lake: Ⅲ. Influence of dredging on denitrification in sediments ZHONG Jicheng, LIU Guofeng, FAN Chengxin, BAI Xiuling, LI Bao, ZHANG Lu DING Shiming (State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, P.R.China) Abstract : A laboratory experiment for studying the effects of sediment dredging on denitrification process was carried out through a one-year incubation of undredged sediments (control) and dredged sediment cores. Denitrification rates in the sediments were estimated by means of the acetylene blockage technique. During the experiments, the denitrification rates in the undredged and dredged sediments ranged from 21.6 to 102.7nmol/(g⋅h) and from 6.9 to 26.9nmol/(g⋅h), respectively. The denitrification rates in the undredged sediments were significantly higher (P 0.05) than those in the dredged sediments throughout the incubation period, with an exception of February 2006. The relative importance of various environmental factors on denitrification wa