5_氮_2_脱氧胞苷对结肠癌细胞生物学行为影响_林洁.pdfVIP

472 临床与实验病理学杂志 J Clin Exp P athol 2008 A ug; 24( 4) 52 林 洁, 黄 琼, 来茂德 : 52( 5aza2deoxycy tid ine, 5a zadC ) SW 620COLO 205 HT29 , 5azadC Annex in V /P opid ium Iod ide T answ ell RTPCR TH BS 1T IM P3RA SSF 1A cmyccfosbax 5azadC 5azadC, 3 G /M , ( P 005) 2 72 h, , 5azadC , (P 005) 5azadC, 5azadC, : ; 52; ; ; : R 73535 : A : 1001- 7399( 2008) 04- 0472- 06 Effects of 5aza2deoxycytidine on biologicalbehavior of colon tumor cell lines L IN Jie, HUANG Q iong, LA I M aode (Departmen t of Pathology, School of M ed icine, Zhej iang Univers ity, H angzhou 3 0058, China) Abstract: Purpose T o investigate the effects of 5aza2deoxycytid ine ( 5azadC ) on the b io log ica l behav io of 3 co lon tum o cell lines, SW 620, COLO 205 and HT29 and to detec t the exp ession changes of som e tum o supp esso genes and oncog enes in these cell lines exposed to 5azadC t eatm ent. M ethods A ll the tumo ce ll linesw e e t eated w ith dem ethy lation agent 5azadC. F low cytom e t y w as app lied to de tect tum o cell cyc le dist ibution. Ce ll apopto sis ana lysis w as pe fo m ed w ith annex in V /p op idium iodide m e thod. M i g ation assay and t answe ll inv asion assay w e e ca ied on to m easu e the m ig ation and invasion ab ilities of SW 620 ce lls w ith o w ithout 5azadC t eatm ent. In addition, RTPCR was ca ied out to ex am ine the exp ession chang es of 3 tum o supp esso genes and 3 onco genes. Results A fte 5azadC t ea tm en t, tumo cell cyc le was a ested atG /M phase. The o ccu ence of apoptosis in 5azadC t eated 2 g oup inc eased s ignificantly. 5azadC a lso sign ificantly inh ib ited the m ig ation and invasion abilities of tumo ce ll lines. T he exp es sions of tum o supp esso genesw e e esto ed wh ile those of oncogenes w e e em a in unchang ed. Conclu