Toll样受体对间充质干细胞免疫调节Th细胞的作用.docVIP

Toll样受体1对间充质干细胞免疫调节Th17细胞的作用 郭振兴1，陈振萍2 1清华大学第一附属医院血液肿瘤科，北京市?100016；2北京儿童医院血液中心，北京市?100045 The role of Toll-like receptor 1 in the immunoregulatory regulation of Th17 cells by mesenchymal stem cells Guo Zhen-xing1, Chen Zhen-ping2 1Department of Hematology/Oncology, First Hospital of Tsinghua University, Beijing 100016, China;?2Hematology Center, Beijing Children’s Hospital Affiliated to Capital Medical University, Beijing 100045, China 摘要? 背景：课题组前期研究表明间充质干细胞具有免疫调节Th17细胞的作用，但内在的调节机制尚待阐明，为此，针对Toll样受体1在其中的作用进行探讨，为今后潜在的细胞治疗策略提供可能的实验依据。 目的：探讨Toll样受体1对间充质干细胞免疫调节Th17细胞的作用。 方法：贴壁法分离人胚胎骨髓来源间充质干细胞，免疫磁珠法分离正常人CD4+?T细胞。CD4+?T细胞单独培养或与间充质干细胞共培养4 d。实时定量聚合酶链反应测定白细胞介素17、Toll样受体1相关基因的表达水平；流式细胞仪检测Th17细胞的数量。 结果与结论：CD4+?T细胞及间充质干细胞均表达Toll样受体1。相对于CD4+单独培养组，间充质干细胞共培养组(间充质干细胞+CD4)白细胞介素17明显升高(3.59±0.11，1.14±0.08，P? 0.01)；进一步发现Toll样受体1 mRNA表达水平亦相应升高(6.07±1.79，1.53±0.63，P? 0.01)。流式细胞仪检测发现，共培养组(间充质干细胞+CD4)Th17细胞数量明显高于CD4+?T细胞组[(4.53±1.27)%，(2.39±0.80)%，P? 0.01)。研究结果表明Toll样受体1可能参与间充质干细胞免疫调节人Th17细胞的作用。 中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程 全文链接： 关键词?：?干细胞；?骨髓干细胞；?Toll样受体1；?骨髓间充质干细胞；?Th17细胞；?免疫调节；?国家自然科学基金； ?? Abstract： BACKGROUND:?Our previous studies have shown that mesenchymal stem cells play an immunomodulatory role in Th17 cells, but the mechanism of action remains to be elucidated, therefore, to explore the role of Toll-like receptor 1 in which will provide possible experimental basis for the future potential of cell therapy strategies.OBJECTIVE: To investigate the role of Toll-like receptor 1 in the immunoregulatory function of mesenchymal stem cells on Th17 cells.METHODS:?Separation of adherent bone marrow-derived human embryonic sources of mesenchymal stem cells, immunomagnetic separation of normal CD4+?T cells. CD4+?T cells were cultured alone or in combination with mesenchymal stem cells co-cultured 4d. Real-time quantitative polymerase chain reaction assay interleukin-17, Toll-like receptor 1 expression levels related genes; number of T