替沙坦对型糖尿病大鼠心功能的影响.docVIP

替沙坦对2型糖尿病大鼠心功能的影响 王小华，殷士良，赵玉萍，刘晓峰，赵春艳，周凤娟 河北省唐山市滦县人民医院 内分泌科 河北 滦县 063700 [摘要] 目的 观察替米沙坦对2型糖尿病大鼠心功能的保护作用，探讨其可能的细胞因子机制。方法 40只雄性SD大鼠随机分为3组：正常对照组(C组)10只，糖尿病模型组(D组)，替米沙坦组(T组)。给予替米沙坦12周末结束实验，检测心功能，免疫组织化学方法检测心肌组织中TGF-β1和HGF蛋白含量。结果 与C组比较，D组，T组LVEDP显著升高(P＜0.01)，-dp/dtmax降低(P＜0.05)；与D组比较，T组LVEDP显著降低，(P＜0.01)，-dp/dtmax升高(P＜0.05)。与C组比较，D组，T组TGF-β1和HGF灰度值降低，与D组比较，T组TGF-β1灰度值升高(P＜0.01)；T组HGF灰度值降低(P＜0.01)。结论 替米沙坦能够改善糖尿病大鼠心功能，可能与降低心肌中TGF-β1，升高HGF表达有关。 关键词 2型糖尿病；心功能；TGF-β1；HGF；替米沙坦 Effects of telmisartan on cardiac function in diabetic rats Wangxiaohua, Yinshiliang, Zhaoyuping, Liuxiaofeng, Zhaochunyan, Zhoufengjuan Dept. of Endocrinology, People Hospital, Luan County，Tangshan CityHebei Province, Luanxian 063700, China Abstract Objective Through investigating the protection to myocardition of telmisartan in diabetic rats, explore cytokine mechanism which telmisartan improve diabetic myocardium. Method Forty male SD rats were randomly divided into three groups: the normal control group (C group), diabetes control group (D group), telmisartan intervention group (T group). The experiment ended until l2 weeks given telmisartan. Heart function were measured and semiquantitative expressions of TGF-β1 and HGF protein were determined by immunohistochemistric method. Results LVEDP of D and T groups were much higher(P0.01) and -dp/dtmax were declined significantly compared wiht C group (P0.05). LVEDP of T was declined significantly (P0.01) and -dp/dtmax was elevated statisically significant compared wiht D group (P0.05). The levels of HGF and TGF-β1 of D and T groups were evated statisically significant compared wiht C group (P0.01), The levels of HGF of T group was evated statisically (P0.01), howerve the levels of TGF-β1 of T group was declined statisically compared wiht D group (P0.01). Conclution Telmisartan can improve the cardiac function of diabetic rats and its protective effect may be related to the reduction of TGF-β1 and increation of HGF. Key words： Type 2 diabetes melli