血清中m-AST同工酶匀相免疫抑制测定法.docVIP

血清中m-AST同工酶匀相免疫抑制测定法 EVEN PHASE IMMUNOSUPPRESSION METHOD FOR MEASURING M-AST ISOENZYME IN SERUM 刘晓艳1，王勤富1，殷建华2 （1.上海利群医院200333；2.上海利群医院进修生） 摘 要 [目的]研究建立血清中m-AST同工酶匀相免疫抑制测定的方法。[方法]利用抗S-AST的羊抗人抗体，与血清中S-AST形成免疫复合物，抑制S-AST活性，建立自动分析仪速率法测定血清中m-AST活力，是根据NCCLS相关文件，对方法进行评估。[结果]抗S-AST的抗体10μl抑制S-AST的有效率≥88%，特异性好，对m-AST无交叉抑制。线性在m-AST 100U/L，r=0.9979,批内CV 1.77%~3.92%，批间CV 2.25%~4.13%，日间CV 3.44%~4.89%，总CV 4.87%~5.02%。在与上海长征医院电泳法对照比较，r=0.9976。参考值为5~17U/L。[结论]用匀相免疫抑制法测定血清中m-AST同工酶具有简便快速，特异性重复性好。适用于手工和自动分析仪测定。 关键词 门冬氨酸氨基转移酶；同工酶；m-AST免疫抑制 Even phase immunosuppression method for measuring M-AST isoenzyme in serum Lin Xiaoyan1, Wang Qinfu1, Yin Jianhua2. 1. Shanghai Liqun Hospital, shanghai 200333, China Abstract [Objective] To study and find the even phase immunosuppression method to measure m-AST Isoenzyme in Serum. [Methods] Anti-S-AST goat anti-human antibody forms an immunocomplex with S-AST in serum to inhibit the activity of S-AST. Then found kinetics on auto analyzer to assay the activity of m-AST in serum. And this measuring method was evaluated according to relative NCCL documents. [Results] The efficiency of 10μl anti-S-AST antibody inhibiting S-AST≥88%, good specificity, no cross inhibition. Linearity of m-AST100U/L, r=0.9979, CV intra-lot 1.77%~3.92%, CV inter-lot 2.25%~4.13%, between day CV 3.44%~4.89%, total CV4.87%~5.02%. Compared with the electrophoresis technique of Shanghai Changzheng hospital, r=0.9976. Reference value is 5~17U/L. [Conclusions] The even phase immunosuppression method to assay m-AST Isoenzyme in serum is simple, convenient and fast. Both its specificity and precision are good. It’s applicable for measuring by hand and by auto analyzer. Key Words AST; Isoenzyme; m-AST immunosuppression [中图分类号]R446.6 [文献标识码]A [文章编号]1004-8685（2004）06-580-03 天门冬氨酸氨基转移酶（Aspartate aminotrans- ferase, AST, EC2. 6 . 1 . 1）属细胞内功能酶，Fleis- cher等首次证实了AST有两种同工酶[1]即存在于细胞浆中的胞质天门冬氨酸氨基转移酶（S-AST）和存在于线粒体内的线粒体天门冬氨酸氨基转移酶（m-AST）。这两种功酶广泛存在于动物或人的肝、心、骨骼肌和肾等组织中，尤以心、肝含量