SSR和ISSR分子标记及在植物遗传育种的研究中应用.pdfVIP

25 1 Vol.25 No. 1 2002 1 Journal of griculturalUniversity of Hebei Jan. 2002 : 1000- 1573(2002) 01- 0090- 05 SSR ISSR 张立荣, 徐大庆, 刘大群 ( , 071001) : SSR(Simple Sequence Repeat) 和ISSR ( Inter- Simple Sequence Repeat) 技术是在PCR 基础上发展起来的两种DN 多态性检测技术, 已开始 用于基因组研究的各 个领域概述了SSRISSR 反 的原理特点, 总结了其在植物亲缘 关系和遗传多态性研究DN 指纹库的建立遗传图谱的构建和基因定位及分子标记辅助育种等方面的 用, 并肯 定了SSRISSR 在植物遗传育种领域的广阔 用前景 : SSR;ISSR;分子标记; 遗传育种 : S 435 : SSR marker, ISSR marker and their application to plant genetics and breeding ZHANG Lirong, XU Daqing, LIU Daqun (College of Plant Protection, gricultural University of Hebei, Baoding 071001, China) Abstract :Simple sequence repeats (SSR) and Inter- simple sequence repeats ( ISSR) are two kinds of DN markers based on thepolymerase chain reaction (PCR) . They have been applied in many aspects of genome re search. This paper has clarified the theories and characteristics of SSR as well as ISSR. The authers also sum marized their applications in geneticpolymorphisim andgeneticrelationship, establishment of DN fingerprinting pool, construction of geneticmap, genelocalization and marker- aided selection. Thetwo methods of DN ec ular marker open up broad prospects for the studies of plant genetics and breeding. Key words: SSR; ISSR; molecular marker; genetics breeding ,, , DN ( RFLP) DN (R PD) ( FLP) (SC R) (VNTR) (SSR) (IS SR) SSRISSR 1 SSRISSR 分子标记技术的原理和特点 1. 1 1~ 4 (Simple sequence repeat SSR) : 2001- 03- 22 : 河北省自然科学基金资助项目(397099) : 张立荣( 1973- ) , 女, 天津市人,在读硕士生, 主要从事分 植物病理学研究. 1 :SSRISSR 91 [1] (Microsatellite) , (G )n( C)n(G