[T-RFLP技术在胃肠道上应用]Monitoring of Antibiotic-Induced Alterations in the Human Intestinal Mic.pdfVIP

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Jan. 2005, p. 501–506 Vol. 71, No. 1 0099-2240/05/$08.00 0 doi:10.1128/AEM.71.1.501–506.2005 Copyright © 2005, American Society for Microbiology. All Rights Reserved. Monitoring of Antibiotic-Induced Alterations in the Human Intestinal Microflora and Detection of Probiotic Strains by Use of Terminal Restriction Fragment Length Polymorphism Cecilia Jernberg,1,2 ˚ 2 1,2 3 Asa Sullivan, Charlotta Edlund, and Janet K. Jansson * Section for Natural Sciences, So¨derto¨rn University College, Huddinge,1 Department of Laboratory Medicine, Karolinska Institute, Stockholm,2 and Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala,3 Sweden Received 29 April 2004/Accepted 29 August 2004 Terminal restriction fragment length polymorphism (T-RFLP) was investigated as a tool for monitoring the human intestinal microflora during antibiotic treatment and during ingestion of a probiotic product. Fecal samples from eight healthy volunteers were taken before, during, and after administration of clindamycin. During treatment, four subjects were given a probiotic, and four subjects were given a placebo. Changes in the microbial intestinal community composition and relative abundance of specific microbial populations in each subject were monitored by using viable counts and T-RFLP fingerprints. T-RFLP was also used to monitor specific bacterial populations that were either positively or negatively affected by clindamycin. Some dominant bacterial groups, such as Eubac- teriu