ING4基因表达上调对卵巢上皮性癌OVCAR细胞顺铂耐药性的影响.pdfVIP

·1254 · 张 芳，等 ING4基因表达上调对卵巢上皮性癌OVCAR细胞顺铂耐药性的影响 ING4基因表达上调对卵巢上皮性癌 OVCAR细胞顺铂耐药性的影响 张 芳 ，柳英兰1 2 Ｃｉｓｐｌａｔｉｎ ｒｅｓｉｓｔａｎｃｅ ａｆｆｅｃｔｅｄ ｂｙ ＩＮＧ4 ｃａｎｃｅｒ ｉｎｃｒｅａｓｅｓ ｇｅｎｅ ｅｘｐｒｅｓｓｉｏｎ ｉｎ ｅｐｉｔｈｅｌｉａｌ ｏｖａｒｉ- ａｎ ｃａｎｃｅｒ Zhang Fang ，Liu Yinglan1 2 1 2 Department of Urology，LiaoyuanMining Group GeneralHospital，JilinLiaoyuan 136201，hina；Departmentof Obstetrics and Gynecol- ogy，The FirstAffiliated Hospital of HarbinMedical University，Heilongjiang Harbin 150001，hina. 【Abstract】 Objective：To investigate the ING4 gene upregulation of epithelial ovarian cancer ovarian cancer ）on cisplatin -resistant cell lines OVCAR cells.Methods：The liposome -mediated ING4 -Ad and the corresponding negative control fragment was transfected into human ovarian cancer OVCAR，rats were divided into three groups： transfection group，negative control group andblank control group.Real-timePCRtechnologywasusedto detect the expression of ING4 gene.Using tetrazolium blue MTT）assay to make inhibition of cell proliferation and cell growth curve，colony formation assay measured the growth of the cellsability.Results：In transfection group，negative control group and blank control group ING4 expression of OVCAR cellswere66.76 ± 11.40，1.28 ± 0.09，1.22 ± 0.08 P＜0.01）.MTT assay showed that the growth rate slowed transfected cells，decreased sensitivity to cisplatin，in transfected cellgrowthinhibitionratewasthelowest P＜0.01）.Transfectedcellsgrow slowlycomparedwiththeneg- ative control group，settransfectedcellcolonyformationrateislowerthanthenegativecontrolgroupandblankcontrol group P＜0.05）.Flow cytometry showedthatthethreegroupswiththeextensionof theapoptosisrateincreasedcon- centrations of cisplatin P＜0.05）.Conclusion：Theovarian OVCARcellsING4 gene，inhibit cellproliferation，apop- to