沉默Ku70后人耐药肺癌细胞凋亡基因组表达变化及临床意义.pdfVIP

doi：10．3969／j．issn．1000-484X．2010．04．006 沉默Ku70后人耐药肺癌细胞凋亡基因组表达变化及临床意义① 李亚荣张捷王珂刘冰(吉林大学白求恩第二临床医院，长春130041) 中国图书分类号R730．52文献标识码A 文章编号1000．484X(2010)04-0312-04 [摘要] 凋亡基因肿瘤坏死因子受体家族成员(TNFRSF 高表达；提示Ku70参与非小细胞肺癌耐药的发生；封闭Ku70可改变耐药肺癌细胞的促／抗凋亡基因组的表达呈促细胞凋亡效 应，提示Ku70可能作为上游调控点参与调控部分促／抗凋亡基因组的表达，通过促凋亡表达效应而逆转细胞耐药。 [关键词]肺癌；细胞耐药；凋亡基因；Ku70；siRNA．Ku70 altered andits siR- The of clinical by expressionapoptosis-relatedgenes significance NA-Ku70 in cancercellline silencingdrug-resistantlung U Ke，UU Second Jilin 130041，China Ya·Rong，ZHANG№，WANGB魄．The Hospitalof University，Changchun theeffectofKu70 onthe of inhumanNSLCcell genes line， [Abstract]Objective：Toinvestigate silencingexpressionapoptosis-related of inhumanA549cellsWaSexamined A549and Ku70mRNA dnJg-resislamA549DDP．Methods：Expression byRT-PCR．Expressionofapopto- sis-relatedwasexamined Ku70mRNAwas inA549DDPcellsthaninA549cells．The genes byPCR-Arrayassay．Results：The higher expression of and increasedwh刨陀a8the ofTNFRSFlAandBFARdecreasedinsiRNA-Ku70A549DDPcells BAX．TP53TP73 w鹅significantly expression that im- totheseofA549DDPcells．C．tmclmion：1he出啪岫tofKu70mRNAis inA549DDP Ku70is when咖lpared higher ceils，suggesting for Ku70 iumCel／F沱the inA549DDPcells of of expression portantdmg-r嘴islallIce；Silencingmight drug-resistance thro,chregulation apoptotic genes·