一株银杏内生真菌鉴定及活性代谢产物与研究(修改稿).docVIP

一株银杏内生真菌的鉴定及其活性代谢产物研究 王国平2，3#，王丽薇1，3#，张亚磊3，4，王佳莹2，3，徐旭辉5，章初龙2.3 （1.杭州师范大学医药卫生管理学院，杭州 310036；2.浙江大化浙大生物药物研发中心，杭州 310029；3.浙江大学生物技术研究所，杭州 310029；4.浙江工业大学药学院，杭州 310029；5. 浙江大学农药与环境毒理研究所，杭州 310029） 浙江自然保护区Ginkgo biloba中分离获得1株活性形态，鉴定Penicillium commune。发酵粗提物采用正相硅胶柱层析和Sephadex LH-20凝胶以紫外光或碘蒸汽显迹，配合活性追踪圆弧菌醛酸yclopaldic acid）。活性测试表明，该化合物具有抗菌活性，对鸭跖草（Commelina suffruticosa），反枝苋（Amaranthus retroflexus），马唐（Digitaria sanguinalis）空心莲子草（Alligator Alternanthera）Oriza sativa L.）等植物均具有致病毒性，对芝麻（Sesamum indicum）和大豆（Glycine max）不敏感，该菌株具有较好的开发应用价值。 关键词：内生真菌；团青霉；活性代谢产物；活性；圆弧菌醛酸 中图分类号482.292，O658.1，O657.2 文献标识码：A Identification of an endophytic fungus of Ginkgo biloba TMSF169 and its antifungal metabolitesWang Guoping 2，3#, Wang Liwei 1，3,Zhang YaLei3,4 , Wang JiaYing 2,3, Xu Xuhui5, Zhang Chulong 1,2* 1. College of Medicine and Public Health Management, Hangzhou Normal University, Hangzhou 310036; 2. Zhejiang Dahua Zhejiang University Biomedicament RD Center, Hangzhou 311616; 3. Institute of Biotechnology, Zhejiang University, Hangzhou 310029；4. College of Pharmaceutical science, Zhejiang University of Technology,Hangzhou 310029; 5. Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, China Abstract: Endophytic fungi in medicinal plants are important sources of microorganisms, which produce a variety of bioactive compounds. In this study, an endophytic fungus TMSF169 with antifungal activity was isolated from Ginkgo biloba in Tianmushan Nature Reserve, Zhejiang. Strain TMSF169 was identified as Penicillium commune based on the morphology and ITS rDNA sequence analysis. Antifungal and herbicidal compounds?were?isolated?from?the?fermentation?broth?of?TMSF169 through normal-phase silica gel column chromatography and gel?(Sephadex?LH-20)?column?chromatography, traced by ultraviolet?light?or?iodine?vapor with bioassay-guided?fractionation. TMSF169A one of these isolated compounds was elucidated as cyclopaldic acid based on mass?spectrometry?and?nuclear?magn