丙泊酚对大鼠海马CA1区突触传递长时程抑制的作用.pdfVIP

· 66 · 医药杂志2010年 01月第 27卷第 O1期 PracJMedPharmVol27，2010—01No．01 丙泊酚对大鼠海马CA1区突触传递长时程抑制的作用 李晓强，孙雪华 摘『 要1 目的 研究丙泊酚对大 鼠海马 CA1区兴奋性突触反应及突触可塑性 的影 响。方法 取 3周龄 Wistar大鼠，快速断头取脑 ，用振动切片机切取 400 m厚 的海 马脑片 ，电刺激靠近海马 CA1区的 Schaffer纤维 ，用 全细胞膜片钳技术记录 CAI区锥体细胞的兴奋性突触后 电流 (excitatorypost—synapticcurrent，EPSC)。循环液中加 人不同浓度 的丙泊酚 ，观察其对 EPSC的影响 。然后给与低频刺激 (900pulse，3Hz)诱导长时程抑制 (1ong—term depression，LTD)，并观察丙泊酚对 LTD诱导的影响。结果 丙泊酚呈剂量依赖性地抑制 EPSC，其作用可被印防己毒 素(pierotoxin)阻断；丙泊酚可易化 由N一甲基一D一门冬氨酸(N—methyl—D—aspartate．NMDA)受体介导的LTD的诱导 。 结论 丙泊酚可影响大鼠海马 CA1 的兴奋性突触传递和突触可塑性，从而对大 鼠的学习和记忆产生影响。 [关键词] 丙泊酚 ；兴奋性突触后 电流 ；长时程抑制 ；海马；学习和记忆 f中图分类号1 R614．24：R一332：Q42 文『献标识码】 A Efficacy ofpropofolon long-term depression in the CA1 region ofrathippocampus LIXiao-qiang ， SUN Xue-hua．~DepartmentofAnesthesiology，No．107 CentralHospitalofPLA，Yantai，SHandong264000，China A【bstract】 Objective Toevaluatetheimpactofpropofolon excitatory synapticresponsesand synaptic plasticity in rathippocampus．Methods Male Wistarrats (3 week old)were decapitated rapidly and the hippocampalbrainsliceswerecutcoronallyin400um．Themicroscopewasusedtoidenti~ theCA1regionof thehippocampus．WithelectricalstimulationonSchaffercollaterals，theexcitatorypost-synapticcurrent(EPSC) were recorded using blind patch—clamp technique；after 10rain recording ofEPSC baseline，propofolwas perfused in bath solution with differentconcentrations．Then EPSC wascontinously recorded foranother30min．The impact of propoofl on synaptic plastisity after 10min recording of EPSC baseline was observed after low ~equency stimulation FS，O．3Hz，900pulse1wasusedtoinduceLTD． Thentheslicewasincubatedwithpropofolofr0。5h and LTD wasinduced asabove．Results Propofoldose—dependently inhibited EPSC，thiseffectcan be completely blocked by picrotoxin． Propofol also facilitated LTD inducti