壳聚糖包被磁性荧光多功能复合粒子的研究.docVIP

壳聚糖包被磁性－荧光多功能复合粒子的研究 chitosan -Coated Luminescent/Magnetic Silica Microspheres research 刘少君 [摘要] 集磁性和荧光于一体的复合型纳米粒子以其良好的应用前景备受关注。本文在磁性Fe3O4纳米颗粒表面包裹SiO2层，制备了Fe3O4@ SiO2复合粒子。复合粒子表面再连接阳离子聚合体羧甲基壳聚糖，通过静电作用，使带负电的量子点吸附于壳聚糖链上，最后将壳聚糖交联，形成稳定的荧光-磁性纳米复合粒子。通过红外、荧光光谱、扫描电镜、动态光散射等不同的分析测试手段对复合粒子性能进行了表征，结果表明成功制备了Fe3O4@SiO2@CMCH/CdTe复合粒子，该粒子是同时具有荧光和磁性的多功能纳米复合微粒，在靶向载药方面有着很广泛的应用。 关键词： 量子点; CdTe ； 羧甲基壳聚糖 ； 磁性纳米粒子 ； 荧光； 载药 [ Abstract ] Because of their great potential application, the multifunctional microspheres combining several advantages of fluorescence and magnetism had attracted great attention from scientists in many fields . In this article, silica-coated Fe3O4 particles were prepared at first, and then carboxymethylated chitosan (CMCH) was connected onto the surface of Fe3O4@SiO2 particles. By the electrostatic interaction between cationic CMCH and negatively charged CdTe quantum dots, CdTe nanoparticles were adsorbed on the chains of CMCH. Finally, Fe3O4@SiO2@CMCH/CdTe particles were obtained via the selective cross-linking of CMCH using glutaraldehyde. The as-prepared particles were characterized by transmission electron micrographs (TEM), fourier transform infrared (FTIR) spectroscopy, fluorescence spectroscopy and dynamic light scattering (DLS). It is indicated that Fe3O4@SiO2@CMCH/CdTe composite microspheres were prepared successfully. This microspheres are of fluorescence and magnetism, which is desired as carriers for diagnostics, target-oriented drug-delivery systems and so on. Key Words： quantum dot; CdTe；carboxymethylated chitosan ；magnetic nanoparticle ；fluorescence ；drug delivery 一、前 言 纳米技术与生物技术融合而成的纳米生物技术是国际生物技术领域的前沿和热点问题，在医药卫生领域有着广泛的应用和明确的产业化前景，特别是纳米药物载体、纳米生物传感器和成像技术以及微型智能化医疗器械等，将在疾病的诊断、治疗和卫生保健方面发挥重要作用。 1.1磁性－荧光多功能复合微球： 利用磁性纳米颗粒制造靶向输送医疗药物，是目前医药学研究的热点。通常的靶向纳米药物载体是运用了载体对机体各组织或病变部位亲和力的不同，或将单克隆抗体与载体结合，使药物能够转运到特定的治疗部位。由于磁性纳米颗粒的粒径比毛细血管通路还小 1~2 个数量级，用其作为定向载体，通过磁性导向系统控制，可将药物靶向输送到病变部位释放，以增强疗效。制备出生物相容性和单分散性较好的无机磁性纳米颗粒载体（主要为铁系氧化物），再用生物高分子（氨基酸、多肽、蛋白质、酶等）包覆磁性纳米颗粒载体，再将包覆好的磁性