低分子肝素预处理对大鼠急性肺损伤的影响.docVIP

低分子肝素预处理对大鼠急性肺损伤的影响 北京大学第一医院麻醉科 邢军 吴新民 【摘要】目的 探讨低分子肝素（LMWH）预处理对急性肺损伤（ALI）大鼠细胞因子和粘附分子表达的影响。方法 腹腔内注射内毒素制备ALI大鼠模型。50只SD大鼠随机数字表法分为正常对照组、ALI组、LMWH预处理组（100u/kg、200u/kg和300u/kg），每组10只。观察肺湿干重比（W/D）、髓过氧化物酶（MPO）活性TNF-α水平、血浆凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）、血浆纤维蛋白原水平以及肺组织ICAM-1E-selectin、组织因子（TF）和纤溶酶原激活物抑制物（PAI）-1 mRNA的表达情况。结果 ALI组的W/D、MPO活性和TNF-α含量、肺组织炎性细胞因子、粘附分子的mRNA表达均明显升高TNF-α含量明显降低（p0.05），但是BALF总蛋白浓度、肺组织ICAM-1和E-selectin的mRNA表达没有明显改变。ALI组和LMWH预处理三组的PT和APTT较盐水对照组有所升高（p0.05），而纤维蛋白原的水平显著降低（p0.05）；与ALI组相比，LMWH200组的PT时间明显降低（p0.05），纤维蛋白原的水平明显升高（p0.05）结论 LMWH预处理能抑制中性粒细胞激活，减低TNF-减轻ALI时的炎症反应。 【关键词】 急性肺损伤；低分子肝素预处理；细胞因子；粘附分子；凝血与纤溶； The effects of low molecular weight heparin pretreatment on acute lung injury in rats Xing Jun, Wu Xin-min. Department of Aneshtesiology, First hospital, Peking University, Beijing 10034, China 【Abstract】 Objective evaluate the effects of low molecular weight heparin (LMWH) pretreatment on preventing rats from acute lung injury induced by lipopolysaccharide (E. Coli O55:B5). Methods Fifty male SD rats were randomly divided into five groups (n=10). Control group, injected with saline intraperitoneally. Lipopolysaccharide (LPS) group, with intraperitoneal injection of LPS 6mg/kg body weight. LMWH 100 group, injected with LMWH 100u/kg subcutaneously 2 hours before LPS injection. LMWH 200 group, injected with LMWH 200u/kg subcutaneously 2 hours before LPS injection. LMWH 300 group, injected with LMWH 300u/kg subcutaneously 2 hours before LPS injection. Four hours after LPS/saline administration, the rats were anesthesized and killed. The lungs wet/dry ratio was determined. Total protein of bronchoalveolar lavage fluid (BALF) was calculated by BCA protein quantity method. MPO and caspase-3 activity of lung tissue were measured spectrophotometrically. The level of TNF-α in lung tissue was quantitated by ELISA. Blood was withdrawed from the right ventricle of each rat. Plasmic prothrombin time (PT), activated partial thromboplastin time (APTT) and fibrinogen lev