中医不同证型帕金森病患者外周血单个核细胞蛋白质组学研究.docVIP

中医不同证型帕金森病患者外周血单个核细胞蛋白质组学研究 作者：区健刚梁清华陈疆曾年菊熊新贵萧梅芳关勇军鄢东红【摘要】：目的中医肝阳化风证和血虚生风证帕金森病患者和人外周血单个核细胞(PB)的蛋白质组差异。从蛋白表达上探讨中医肝阳化风证和血虚生风证… 作者：区健刚 梁清华 陈疆 曾年菊 熊新贵 萧梅芳 关勇军 鄢东红 【摘要】 ：目的 中医肝阳化风证和血虚生风证帕金森病患者和人外周血单个核细胞(PB)的蛋白质组差异。从蛋白表达上探讨中医肝阳化风证和血虚生风证的本质。方法 用固相pH梯度双向凝胶电泳分离肝阳化风证和血虚生风证帕金森病患者和人PB总蛋白质,考马斯亮蓝染色,PDQuest 2-DE软件分析,对差异蛋白质点基质辅助激光解吸电离飞行质谱(ALDI-TF-S)测定其胶内酶解后的肽质指纹图谱,用ast查询系统查询SISS-PRT数据库。结果 了分辨率和重复性均的双向电泳考染图谱,对的15个差异蛋白质点分别肽质指纹分析,经数据库查询,鉴定为与细胞骨架、抗氧化应激、蛋白降解、信号转导、细胞周期调控等的蛋白质。结论 了帕金森病肝阳化风证和血虚生风证PB的双向凝胶电泳图谱,提示帕金森病肝阳化风证和血虚生风证患者和人的PB的蛋白质表达差异,蛋白质组的差异分析有助于为肝阳化风证和血虚生风证本质在蛋白质上物质基础。 【关键词】 肝阳化风 血虚生风 帕金森病 单个核细胞 双向凝胶电泳 质谱 Abstrat：bjetive T investigate the parative prtees differene f PB beteen health adults and Parkinsn Disease (PD) patients ith Ganyang Huafeng syndre and Xuexu Shengfeng syndre. S t apprah the essene f the t T syndres fr the prtein expressin level. ethds A series f ethds, inluding ibillized pH gradient t-diensinal plyarylaide gel eletrphpresis (2-DE), assie Brilliant Blue staining, PDQuest 2-DE analysis sftare, peptide assfingerprint based n atrix-assisted laser desrptin/inizatin tie f flying ass spetretry (ALDI-TF-S) and SISS-PRT database searhing, ere used t separate and indentify the prtee f PB beteen health adults and PD patients ith Ganyang Huafeng syndre and Xuexu Shengfeng syndre. Results The gd 2-DE pattern inluding reslutin and reprduibility as btained. After assie Brilliant Blue staining, 15 spts ere inised and analyzed by ALDI-TF-S. The typi peptide asses ere searhed in the SISS-PRT database by ast sftare. All prteins ere preliinarily identified, hih ralated t ytskeletn, anti-xidative stress, prtein degradatin, signal transdutin and ell yle, et. nlusins The ell-reslved, reprduible 2-DE patterns f Ganyang Huafeng syndre and Xuexu Shengfeng syndre PD ere established and revealed the prtein expressin differene f PB beteen the t syndres f PD patients. This researh is helpful t prvide substane evidene in investigatin f the essene f Ganyang Hua