胎盘Bcl-2、Bx表达与锌拮抗汞对母儿毒性的相关性研究.doc

胎盘Bcl-2、Bax表达与锌拮抗汞对母儿毒性的相关性研究 朱泓1，2，杨祖菁2， 惠宁1*，刘军霞3 1.第二军医大学长海医院妇产科 ，上海 200433 2.上海交通大学医学院附属新华医院妇产科，上海 200092 3.环境与儿童健康教育部和上海市重点实验室，上海 200092 [作者简介] 朱泓，第二军医大学长海医院妇产科在读博士生,上海交通大学医学院附属新华医院妇产科主治医师. Email:wendybonjour@] *通讯作者（corresponding author）. Tel: 021 E-mail: huin1956@163.com [摘要]目的 探讨孕期补锌对母儿汞毒性的拮抗作用及其抗凋亡机制。方法 根据孕中、晚期母血汞值选取病例组、治疗组、对照组孕妇各30例，比较3组孕妇汞、锌水平，妊娠结局，应用蛋白质印迹法分析3组胎盘细胞凋亡相关蛋白Bcl-2、Bax（3.42±0.52 μg/L）（6.16±0.32 μg/L)(P0.05)（671.34±44.65 μg/L）（483.25±62.20 μg/L）(P0.05)(3.42±0.52 μg/L)低于孕中期（6.15±0.26 μg/L）（P0.05）(671.34±44.65 μg/L)高于孕中期（480.73±60.64 μg/L）(P0.05)(OR分别=10.55,5.21,5.09，P均0.05）Bcl-2表达水平(2 055.97±280.82)低于治疗组(5 187.93±247.89）（5 308.83±259.97）(P均0.05)Bax表达水平（3 110.38±136.09 ）（2 015.76±177.63）、（2 009.90±152.61）(P均0.05)，（35.20±4.96%）（13.47±2.43%）、13.27±2.21%）(P均0.05)。Bcl-2表达，下调Bax表达，从而抗凋亡相关。 [关键词]锌；汞；妊娠结局；Bcl-2；BaxCorrelation of placental Bcl-2, Bax expression and Zn antagonism on maternal and fetal toxicity of Hg ZHU Hong 1，2, YANG Zu -jing2 , HUI Ning1* , LIU Jun-xia3 Department of Obstetrics and Gynecology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China 2.Department of Obstetrics and Gynecology, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China 3Moe-Shanghai Key Laboratory of Children’s Environmental Health, Shanghai 200092, China [Abstract] Objective To investigate the protective effect of zinc to the mother-newborn toxicity caused by mercury and its anti-apoptosis mechanism. Methods According to the levels of maternal blood mercury at middle and late pregnancy, 90 pregnant women were equally divided into a case group, a treatment group, and a control group. The levels of maternal blood mercury and zinc, pregnancy outcome, the expression of Bcl-2 and Bax protein in placenta detected by Western-blotting, and the positive rate of apoptosis cells in placenta detected by Tunel were each compared among groups. Results The level of maternal blood mercury