姜黄素对代谢综合征大鼠ICAM_医学论文.docVIP

姜黄素对代谢综合征大鼠ICAM_医学论文 姜黄素对代谢综合征大鼠ICAM_医学论文 作者：陶正贤，龙明智，赵慧娟，谭晓，王迪斌，陶立翠 【关键词】 代谢综合征；细胞黏附分子；姜黄素；内皮功能障碍 　　Effect of curcumin on ICAM1 and relative serum indexes in rats with metabolic syndrome 　　【Abstract】AIM: To investigate the therapetutic effects of curcumin on metabolic syndrome (MS) in rats and the underlying mechanism. METHODS: Thirty SD male rats were randomly separated into 3 groups (n=10 in each one): control, model and curcumin groups. Rats in model and curcumin groups were treated with twokidneys oneclipped to induce renal hypertension and fed with a high fructose diet for 4 week to induce MS. Shamoperated rats were taken at random as noninfarction control. After intragastric administration of either curcumin (200 mg/kg#12539d) in curcumin group or saline (200 mg/kg#12539d) in model and control groups for 4 weeks, serum glucose, insulin and lipid were tested. The sICAM1 level was measured by ELISA and ICAM1 mRNA level in artery was tested by RTPCR. RESULTS: Compared with controls, rats in model group had higher levels of sICAM1, serum glucose, insulin, lipid (0.01), and curcumin restored these indexes. The ICAM1 mRNA expression in model group was higher than that in shamoperated rats (0.01) while in curcumin group, ICAM1 mRNA level was comparable to that in control group (Pgt0.05). CONCLUSION: Curcumin reduces serum glucose, insulin and lipid of MS rats and it may be related to the suppression of ICAM1 mRNA expression and the improvement of blood vessel endothelium function. 　　【Keywords】 metabolic syndrome cell adhesion molecules curcumin endothelial dysfunction 　　【摘要】目的: 探讨姜黄素对代谢综合征（MS）大鼠模型的治疗作用及其机理. 方法: 将清洁级雄性SD大鼠30只随机分为三组：正常对照组，模型组，姜黄素组，每组10只. 模型组、姜黄素组施以两肾一夹术后普通饲料喂养4 wk，诱发肾性高血压，继以高果糖饲料喂养4 wk，诱导建立代谢综合征模型；正常对照组施以假手术. 姜黄素组用姜黄素（200 mg/kg#12539d）灌胃给药，模型组和正常对照组灌等量的生理盐水. 用药4 wk后，检测血脂、血糖等血清学指标，用ELISA法测定血清sICAM1的水平，用RTPCR检测血管内ICAM1 mRNA水平. 结果: 模型组大鼠血脂、血糖等血清学指标和血清sICAM1水平明显高于正常对照组大鼠，差异有统计学意义（0.01）；血管内ICAM1 mRNA水平模型组高于正常对照组，差异有统计学意义（0.01）；姜黄素组大鼠血脂、血糖等血清学指标和sICAM1水平明显低于