妊娠高血压综合征与一氧化氮代谢及氧化应激的关系_医学论文.docVIP

妊娠高血压综合征与一氧化氮代谢及氧化应激的关系_医学论文 妊娠高血压综合征与一氧化氮代谢及氧化应激的关系_医学论文 作者：单伟颖，谢延香，王丽冬，刘亚莉，封桂英 【关键词】 ,妊娠高血压综合征 　　【Abstract】 AIM: To study the correlation between pregnancyinduced hypertension and the metabolism of nitric oxide and the oxidative stress. METHODS: Sixty pregnant women with pregnancyinduced hypertension (PWPIH) and 60 pregnant women with normotension (PWN) were enrolled in a casecontrol study, in which the concentrations of plasma nitric oxide (NO) and the erythrocyte malondialdehyde (MDA) as well as the activities of erythrocyte superoxide dismutase (SOD) and glutathione peroxidase (GPX) were determined by spectrophotometry. RESULTS: NO ［(269±78) nmol/L］, SOD ［(1812±138) kat/g］ and GPX ［(19±5) kkat/g］ in the PWPIH group were lower than those ［(348±94) nmol/L, (2088±152) kat/g and (27±7) kkat/g］ in the PWN group (0.01), while MDA ［(33±3) nmol/g］ in the PWPIH group was higher than that ［(27±3) nmol/g］ in the PWN group (0.01). The findings of bivariate analysis showed that with both decreased NO, SOD and GPX and increased MDA, the systolic blood pressure (SBP) and diastolic blood pressure (DBP) of PWPIH gradually increased (0.01). The findings of stepwise regression analysis suggested that the changes of NO and MDA were most closely correlated with SBP and DBP of PWPIH. The findings of reliability analysis for the above parameters showed that the reliability coefficient (alpha) was 0.6923 (0.001) and that the standardized alpha was 0.9237 (0.001). CONCLUSION: The findings in the present study suggest that significantly decreased NO and increased MDA were most probably the risk factors causing pregnancyinduced hypertension. 　　【Keywords】 pregnancyinduced hypertension syndrome nitric oxide superoxide dismutase glutathione peroxidase malondialdehyde 　　【摘要】 目的: 探讨妊娠高血压综合征（PIH）与一氧化氮(NO)代谢及氧化应激的关系. 方法: 采用病例对照研究，使用分光光度分析法对60例PIH孕妇（PWPIH）与60例正常血压孕妇（PWN）的血浆NO浓度、红细胞超氧化物歧化酶（SOD）活性、红细胞谷胱甘肽过氧化物酶（GPX）活性和红细胞丙二醛（MDA）浓度进行了检测. 结果: 与PWN组的各实验参数平均值比较，PWPIH组的NO平均浓度、SOD和GPX平均活性显著降低（0.01），