心得力对心肌病大鼠心功能及心脏连接蛋白43表达的影响_医学论文.docVIP

心得力对心肌病大鼠心功能及心脏连接蛋白43表达的影响_医学论文 心得力对心肌病大鼠心功能及心脏连接蛋白43表达的影响_医学论文 作者：刘平，程功，强华，韩克，吴格如，马爱群 　　【Abstract】AIM: To investigate the effects of Xindly on heart function and myocardial connexin 43（Cx43）protein expression in doxorubicinlar basis for treatment of cardiomyopathy. METHODS: Fifty male SD rats were randomly divided into 4 groups: normal group, placebo group, imidapril group and Xindly+imidapril group. After intervention for 4 weeks, ejection fraction(EF),left ventricular diastolic dimension(LVDD) and left ventricular endsystolic dimension(LVDS)were measured by Hp Sonos 2500 ultrasound inspectoscope FITClabeled antiCx43 antibody was used in immunohistochemical technology with a quantitative imaging system. RESULTS: The heart function was significantly improved in xindly+imidapril group than in placebo group. LVDS was better in xindly+imidapril group than in imidapril group(0.05). The Per Area, Area, integrated optical density(IOD) of myocardial Cx43 in xindly+imidapril group were better than that in placebo group (0.05). The Per Area in xindly+imidapril group was better than those in imidapril group(0.05). CONCLUSION: Xindly+imidapril administration is effective for the treatment of cardiomyopathy. 　　【Keywords】 cardiomyopathies heart function connexin 43 xindly 　　【摘要】目的： 研究心得力(Xindly)对阿霉素(ADR)心肌病大鼠心功能及心脏连接蛋白43（Cx43）的影响，部分探讨其治疗心肌病的分子基础. 方法： SD大鼠50只随机分为正常组，ADR心肌病心衰模型组(含安慰剂组，达爽组和心得力+达爽组). 药物干预4 wk用Hp Sonos 2500型多功能超声检查仪无创检测各组大鼠心脏射血分数（EF），左心室舒张末内径（LVDD），左心室收缩末内径（LVDS）；FITC标记抗Cx43的免疫组化法染色，激光共聚焦显微镜测量Cx43荧光强度，并作图像定量分析. 结果： 心得力+达爽组心功能较安慰剂组改善，左室收缩末内径较达爽组改善（0.05）. 心得力+达爽组Cx43的Area, Per Area, IOD均好于安慰剂组(0.05)；心得力+达爽组Cx43的Per Area与西药组有统计学差异(0.05). 结论： 心得力+达爽治疗心肌病有疗效. 　　【关键词】 心肌疾病；心脏功能；连接蛋白43；心得力 　　0引言 　　扩张型心肌病(dilated cardiomyopathy, DCM)是一种以心腔［左心室和(或) 右心室］扩大、心肌收缩功能障碍为主要特征的原因不明的心肌疾病,即使在 ACEI 和β受体阻滞剂使用率较高、总体生存率已改善的情况下，恶性室性心律失常所导致的猝死仍是DCM患者除心力衰竭以外的重要死亡原因［1-2］. 虽然离子通道的功能改变是心律失常发生的一个重要因素，但不正常的细胞耦联有时起主要作用［3］. 近年的研究表明，由GJ（gap junction, GJ）通道介导的心肌细胞间电耦联对心脏的电传导速度和传导的各向异性起着更为