慢性庆大霉素损伤后豚鼠前庭感觉上皮蛋白激酶C表达和活性_医学论文.docVIP

慢性庆大霉素损伤后豚鼠前庭感觉上皮蛋白激酶C表达和活性_医学论文 慢性庆大霉素损伤后豚鼠前庭感觉上皮蛋白激酶C表达和活性_医学论文 作者：马晓莉　姜鸿彦　黄维国　邱建华　王锦玲　刘顺利 【关键词】 庆大霉素 　　关键词: 庆大霉素前庭感觉上皮蛋白激酶C豚鼠 　　摘 要:目的 研究庆大霉素慢性损伤后豚鼠前庭终器蛋白激酶C（PKC）活性的变化及其βⅠ ，βⅡ ，γ亚型的表达，探讨PKC与前庭损伤修复的关系. 方法 采用免疫细胞化学法观察椭圆囊斑、球囊斑和壶腹嵴PKCβⅠ，Ⅱ ，γ亚型的表达应用γ-32 P示踪检测前庭终器PKC活性的变化. 结果 PKCβⅠ，Ⅱ ，γ亚型在前庭感觉上皮细胞均呈阳性表达，上皮下结缔组织呈阴性表达.βⅡ ，γ亚型较β1 亚型表达稍弱，不同亚型抗原分布不完全相同.与对照组相比，PKCβⅠ ，γ在停止注射庆大霉素后1d其表达明显减弱，随后其表达逐渐增加，至1wk其表达已高于对照组，3wk仍未恢复.PKCβⅡ 表达各组变化不明显.豚鼠前庭终器PKC活性检测结果与PKCβⅠ ，γ表达变化规律基本相似. 结论 庆大霉素慢性中毒后豚鼠前庭感觉上皮PKCβⅠ ，γ亚型表达及PKC活性均发生变化，且不同亚型抗原分布不完全相同.提示PKC在前庭感觉上皮损伤修复过程中发挥重要作用，不同亚型可能具有不同的作用. 　　Keywords:gentamicinsvestibuleepitheliumprotein ki-nase Cguinea pigs 　　Abstract:AIM To investigate the PKC expression and ac-tivity after chronic gentamicin toxicity in vestibular terminal　organs of guinea pigs and to study the relations between PKC and injury repair process of vestibule.METHODS By using immunohistochemical method，the expressions of PKCβⅠ and PKCγwere examined in ginea pig macula utriculus，macula sacculus and crista ampullaris.Theγ-32 P was used as a tracer to measure the activity of PKC in vestibular terminal organs.RESULTS The positive expression of PKC isoformsβⅠ，Ⅱ andγwas detected in vestibular sensory epithelium.There was negative staining in connective tissue lying under epithe-lium.The stain of PKC isoformsβⅡ andγwas weaker than that of PKCβⅠ .The antigen distributions of different PKC isoforms were different.In comparison with control group，the expression of PKCβⅠ andγdecreased at1d after injection of gentamicin and then it increased gradually.The expression was obviously higher than control group at1wk after injec-tion and it still did not recover at3wk after injection.There were no obvious differences in expressions of PKCβⅡ among different groups.The change trend of PKC activity was simi-lar to the expression change of PKCβⅠ andγas a whole.CONCLUSION There were some changes of PKC expres-sion and activity in vestibular terminal organs of guinea pigs after chronic gentamicin