抱茎蓼花的挥发油成分及其抗菌活性的研究_药学论文.docVIP

抱茎蓼花的挥发油成分及其抗菌活性的研究_药学论文 抱茎蓼花的挥发油成分及其抗菌活性的研究_药学论文 作者：田光辉，刘存芳，赖普辉 【摘要】 　　目的提取野生抱茎蓼花中的挥发油，分析挥发油的组分，探讨其生物活性，为开发这一资源提供理论依据。方法用水蒸气蒸馏法从抱茎蓼的花中提取挥发油，用气相色谱-质谱联用技术对挥发油组分进行分离和鉴定，运用气相色谱面积归一化法确定各组分的相对含量，并利用正构烷烃系列物质对各组分进行定性确定，对抱茎蓼花的挥发油进行抗菌实验。结果从抱茎蓼花的挥发油中检出69个组分，鉴定出66个组分，占全油的96.92%；抱茎蓼花的挥发油有明显地抗菌活性。结论 抱茎蓼花的挥发油中主要以单萜和倍半萜为主，含量较高的组分是石竹烯（12.01 %）、3-己烯-1-醇(10.78%)、α-里哪醇(6.88%)、3-辛烯-3-醇（6.32%）等，其挥发油对大肠埃希菌、伤寒沙门菌、肠炎沙门菌和金黄色葡萄球菌有明显地抑制和灭活作用。 【关键词】 抱茎蓼；抱茎蓼花；挥发油；气相色谱-质谱法；抗菌活性 　　Abstract：ObjectiveTo extract and analyze the constituents of the essential oil from the flower of Polygonum amplexicaule, so as to provide scientific proof for its exploitation of biological activity compositions. MethodsThe essential oil from the flower of Polygonum amplexicaule was extracted by steam distillation, the components of the essential oil were separated and structurally identified by gas chromatography/mass spectrometry, the relative contents of these components by the peak-area normalization method adopted in gas chromatography, and all components of the essential oil are calibrated with a standard mixture of homologous n-alkane series. The essential oil was investigated by antimicrobial activities. Results69 components were separated, 66 components were identified and accounted for 96.92 % of the all peak area, the essential oil had antimicrobial activity. ConclusionThe main chemical constituents of the essential oil are monoterpenoids and sesquiterpenoids compounds, the major components are Caryophyllen (12.01 %), 3-Hexen-l-ol (10.78 %), α-Linalool (6.88%), 3-Octen-3-ol(6.32%), etc. The essential oil has obvious inhibitory effect against Escherichia coli, Salmonella typhi, Salmonella enteritidis and Staphylococcus aureus. 　　Key words：Polygonum amplexicaule; Flower; Essential oil; Gas chromatography-mass spectrometry; Antimicrobial activity 　　陕西南部秦巴山区野生的抱茎蓼Polygonum amplexicaule D.Don是蓼科Polynonaceae蓼属Polygonum L.的一种草本植物，又名红三七［1］。蓼属植物具有清热解毒、散结消肿、活血止痛、顺气解痉、收敛止泻等功效，有些还具有抗菌消炎、抗氧化、抗肿瘤、杀虫等多种生物活性［2］，因此对该属植物生物