杜仲对去势大鼠股骨重、矿物含量、胫骨抗弯力及血清碱性磷酸酶的影响_药学论文.docVIP

杜仲对去势大鼠股骨重、矿物含量、胫骨抗弯力及血清碱性磷酸酶的影响_药学论文 杜仲对去势大鼠股骨重、矿物含量、胫骨抗弯力及血清碱性磷酸酶的影响_药学论文 作者：蔡建平， 张贤， 夏树林， 谢焕松 【摘要】 　　目的观察中药杜仲对去势大鼠股骨重、矿物含量、胫骨抗弯力及血清ALP的影响。方法80只4月龄雌性SD大鼠随机分成4组：假手术组、模型组(阴性对照组)、阿尔法骨化醇（ALF）组(阳性对照组)和杜仲组(实验组)。除假手术组打开腹腔，暴露卵巢后即缝合外，其他3组均摘除双侧卵巢。手术后饲喂10 d，阳性对照组和实验组分别用ALF软胶囊或盐杜仲冲剂灌胃，1次/d，另外2组正常饲养。3个月后，取各组股骨测定股骨重、矿物含量，取胫骨测定抗弯力，取血清测定碱性磷酸酶(ALP)活性。结果与假手术组比较，模型组的股骨重显著降低(0.05)，而阳性对照组和实验组股骨重无明显变化(Pgt;0.05)；实验组股骨矿物含量、ALP活性和胫骨抗弯力显著高于模型组(0.05)，阳性对照组ALP活性显著低于模型组(0.05)和假手术组(0.01)。结论盐杜仲可抑制骨吸收，提高骨的强度，对去势大鼠骨质疏松具有一定的防治作用。 【关键词】 去势大鼠 杜仲 抗弯力 碱性磷酸酶 　　Abstract：ObjectiveTo observe the effect of Eucommia Bark on weight of femur, mineral content, anti-bend strength of tibiae and serum ALP in ovariectomized (ovx) rats. Methods80 femal SD rats were divided into 4 groups: sham, negatire, positive, and experimental groups. The rats in each group were ovariectomized except sham operation group. 10 days after operation, intragastric administration was used to rats in positive and experimental groups with Alfacalcidol (ALF) and Eucommia Bark respectively once a day. After 3 months, femurs were separated and weight and mineral content were determined, and anti-bend strength of tibiae and serum alkaline phosphatase (ALP) were measured in each group. ResultsThe weight of femur in experimental group was decreased obviously (0.05), and positive and experimental groups had no significant change (Pgt;0.05) as compared with sham group. In experimental group, mineral content，activity of serum ALP and anti-bend strength of tibiae were increased compared to negative group (0.05), meanwhile activity of ALP in positive group was lower than negative(0.05) and sham groups (0.01). ConclusionEucommia Bark can inhibit bone absorption and elevate the bone strength, and has protetive and therapeutic effects on osteoporosis of ovariectomized rats. 　　Key words：Ovx rat; Eucommia Bark; Anti-bend strength; ALP 　　骨质疏松是一个世界范围的、日趋严重的影响健康的重要问题。因骨质疏松引起的严重后果——骨折和巨额的医疗费用受到了社会的高度重视［1］。十多年来，国内外开展了中医中药及中西医结合防治骨质疏松症的研究，取得了一定的进展，但大多数研究是成方