枇杷叶中齐墩果酸和熊果酸含量的毛细管电泳高频电导法测定_药学论文.docVIP

枇杷叶中齐墩果酸和熊果酸含量的毛细管电泳高频电导法测定_药学论文 枇杷叶中齐墩果酸和熊果酸含量的毛细管电泳高频电导法测定_药学论文 【摘要】 目的建立枇杷叶中齐墩果酸和熊果酸的含量测定方法。方法采用毛细管区带电泳，高频电导检测;未涂层弹性融硅石英毛细管柱55 cm×75 μm ID，有效长度46 cm;1.2 mmol·L-1三乙胺-HCl(pH=10.60)，0.24 mmol·L-1β-环糊精为运行缓冲液;分离电压12.5 kV;重力进样10 s(高度20 cm)。结果以布洛芬为内标，齐墩果酸、熊果酸线性范围分别为5.1～102.0 g/ml(r=0.999 7)和5.55～111.0 g/ml(r=0.999 3);平均回收率分别为96.0%和97.2%。结论该法简便、准确、快速、重现性好，可用于枇杷叶的质量控制研究。 【关键词】 毛细管电泳; 高频电导检测; 枇杷叶; 齐墩果酸; 熊果酸 　　Abstract：ObjectiveTo establish a method for the determination of oleanolic acid and ursolic acid isomers in Folium eriobotryae. 　　MethodsIt was studied by Capillary Zone Electrophoresis with high frequency conductivity detection. The separation was performed on a uncoated fused silica capillary of 55 cm×75 μm ID(46 cm effective length). 1.2 mmol·L-1 Triethylamine(TEA) buffer containing 0.24 mmol·L-1 β-cyclodextrin and pH adjusted to10.60 with Hydrochloric acid was selected for the running buffer. The voltage applied was 12.5 kV and temperature was maintained at 25℃. The sample was injected by gravity(10 s，20 cm). 　　ResultsThe linear ranges of determination for oleanolic acid and ursolic acid were 5.1～102.0 g/ml(r=0.9997) and 5.55～111.0 g/ml(r=0.999 3). The average recovery for oleanolic acid and ursolic acid was 96.0%,97.2% respectiviely. 　　ConclusionThe method is reliable，simple，rapid and accurate. The method can be used for quantitative determination of oleanolic acid and ursolic acid isomers in Folium eriobotryae. 　　Key words：Capillary Zone Electrophoresis; High frequency conductivity detection; Folium eriobotryae; Oleanolic acid; Ursolic acid 　　枇杷叶为蔷薇科植物枇杷Eriobotrya japonica(Thunb.) Lindl. 的干燥叶，有清肺止咳、降逆止呕之功效，主治肺热咳嗽，气逆喘急，胃热呕逆，烦热口渴等症[1]，主产于广东、江苏、福建、江西等地。近年来对其化学成分的研究表明，其主要成分为三萜酸和倍半萜及其苷类，药理活性的报道也主要集中于三萜酸的抗炎、降血糖和抗病毒活性[2～4]方面。目前枇杷叶的含量测定方法有薄层色谱法[5]和HPLC法[6]。本文采用毛细管区带电泳(Capillary Zone Electrophoresis,CZE)高频电导(High frequency conductivity detection)检测的方法[7]，对不同产地枇杷叶中齐墩果酸(oleanolic acid，简称OA)和熊果酸(ursolic acid，简称UA)进行含量测定的研究，以期为药材质量控制提供可借鉴的依据。 　　1 仪器与试药 　　CES2003毛细管电泳仪，包括高压电