本草消渴丹对实验性糖尿病大鼠糖代谢酶活性的影响_药学论文.docVIP

本草消渴丹对实验性糖尿病大鼠糖代谢酶活性的影响_药学论文 本草消渴丹对实验性糖尿病大鼠糖代谢酶活性的影响_药学论文 作者：杨宏莉　张宏馨　李兰会　王燕　卢淑兰　孙淑敏　张伟伟 【摘要】 目的探讨中药复方—本草消渴丹对2型糖尿病大鼠糖代谢的关键酶己糖激酶、苹果酸脱氢酶活性的影响，为临床用药提供科学依据。方法选择高脂、高糖饲料加小剂量链脲佐菌素（STZ，25 mg/kg，腹腔注射）的方法建立大鼠2型糖尿病模型，分别以本草消渴丹和西药二甲双胍进行干预，每周末断尾取血检测空腹血糖值。治疗4周后检测大鼠空腹血糖值、肌肉己糖激酶（HK）和肝脏苹果酸脱氢酶活性（MDH）。结果本草消渴丹治疗可以显著降低糖尿病大鼠血糖，其肌肉组织HK、肝脏组织MDH活性与模型组对比有显著性差异。结论中药复方—本草消渴丹降低血糖的作用机制之一可能是直接或间接提高了葡萄糖氧化分解的酶活性；本草消渴丹对对抗糖尿病体重降低有一定作用。 【关键词】 本草消渴丹；糖尿病；糖代谢；己糖激酶；苹果酸脱氢酶 　　Abstract：ObjectiveTo study the effect of Chinese Herbal Compound—Bencao Xiaoke pill on the activity of key enzymes - hexokinase and malate dehydrogenase in the carbohydrate metabolism of type 2 diabetic rats and provide scientific basis for clinical application.MethodsType 2 diabetic rat model was built by small dose injection of streptozotocin (STZ) (25 mg/kg, intraperitoneal injection ) and intake of high lipid die. After the model was established, the rats with diabetes were treated with Compound—Bencao Xiaoke pill and metformin.Blood glucose was detected every weekend by cutting rats tails. After four weeks treatment the blood glucose, activities of muscle hexokinase（HK）and liver malate dehydrogenase（MDH） were tested. ResultsThe blood glucose was significantly decreased as compared with model groups, and there had significant differences in the activities of HK of the muscle tissue and MDH of the liver.ConclusionOne of the hypoglycemic mechanism for materia medica Xiaoke pills is to increese directly or indirectly the activity of carbohydrate metabolic enzymes.The Compound—Bencao xiaoke pill has certain effect on the weight loss of diabetic body. 　　Key words：Compound—Bencao xiaoke pill; Diabetes; Carbohydrate Metabolism; Hexokinase; Malate dehydrogenase 糖尿病是一种常见的内分泌系统代谢障碍性疾病，其发病率有逐年上升趋势，其特征性表现为高血糖和糖尿。西医方面虽有多种治疗方法和药物，但由于西药的毒副作用，使西药在糖尿病的治疗方面未能取得突破性进展。近年来，中医药在防治糖尿病却表现出巨大的潜力和光明前景，无论是单味中药还是中药复方，在治疗糖尿病方面均显示出很大的优势［1，2］，本实验以验方生地、山药、枸杞和山萸肉为基础经提取制成本草消渴丹，通过干预2型糖尿病大鼠模型观察其对2型糖尿病大鼠血糖和糖代谢酶活性的影响，为临床用药提供科学依据。 　　1 材料与仪器 　　1.1 药物 　　将复方中药（主要成分：生地20 g，