染料木黄酮与顺铂联用对耐药卵巢癌细胞SKOV_医学论文.docVIP

染料木黄酮与顺铂联用对耐药卵巢癌细胞SKOV_医学论文 染料木黄酮与顺铂联用对耐药卵巢癌细胞SKOV_医学论文 作者：袁鹏，黄艳红，辛晓燕，宋晖，田爽，王德堂 【关键词】 染料 　　bined with cisplatin on drugresistant ovarian carcinoma cell SKOV3 　　【Abstract】 AIM: To investigate the effects of genistein combined with cisplatin on the proliferation and apoptosis of drugresistant ovarian carcinoma cell SKOV3. METHODS: The growth inhibitory effect of genistein and cisplatin on SKOV3 cells was studied by MTT growth assay, the morphological alterations of SKOV3 cells were demonstrated by Hoechst 33342 staining under a fluorescence microscope and apoptosis and cell cycle distribution were detected by flow cytometry. RESULTS: Various concentrations of genistein in combination with cisplatin resulted in the reduction of IC50 by 32.3%-79.8% in SKOV3 cells. The combination index of genistein and cisplatin in SKOV3 cells ranged from 0.586 to 0.869, which indicated that they acted in a slightly to highly synergistic pattern. The apoptotic rate was also significantly raised by combination of genistein and cisplatin, which showed a relationship with G2/M arrest and the disturbance of S phase because there was a negative correlation between apoptotic rate and G1 phase cell fraction（rs=-0.953, 0.05）. CONCLUSION: Genistein can significantly improve the growth inhibition and cytotoxicity effects of cisplatin on SKOV3 cells and the two agents act in a synergistic way. These synergistic effects may be related to the G2/M arrest and the disturbance of S phase by genistein. 　　【Keywords】 genistein cisplatin ovarian neoplasms drug synergism 　　【摘要】 目的：探讨染料木黄酮与顺铂联用对耐药卵巢癌细胞系SKOV3增殖与凋亡的影响. 方法：采用MTT比色法检测染料木黄酮和顺铂联用对SKOV3细胞增殖的影响，Hoechst染色荧光显微镜下观察联合用药后细胞形态的改变，流式细胞仪分析细胞周期并检测凋亡率. 结果：联用不同浓度的染料木黄酮后顺铂对SKOV3细胞IC50降低率为32.3%~79.8%,两药联合作用系数在0.586~0.869之间，为轻度至高度协同作用. 染料木黄酮与顺铂联用可显著提高凋亡率，两药联用诱导SKOV3细胞凋亡和阻滞G2/M期、干扰S期进程，凋亡率与G1期细胞比例呈负相关（rs=-0.953, 0.05）. 结论: 染料木黄酮可以增强顺铂对SKOV3细胞的增殖抑制作用和杀伤作用，两种药物具有协同作用. 染料木黄酮阻滞细胞于G2/M期并同时干扰S期进程可能是两药发挥协同作用的重要机制. 　　【关键词】 染料木黄酮；顺铂；卵巢肿瘤；药物协同作用 　　0引言 　　近年来染料木黄酮（4，5，7三羟基异黄酮