模拟推拉动作对大鼠血小板粘附聚集和血液流变学的影响_医学论文.docVIP

模拟推拉动作对大鼠血小板粘附聚集和血液流变学的影响_医学论文 模拟推拉动作对大鼠血小板粘附聚集和血液流变学的影响_医学论文 作者：沙建慧, 朱洪鸣 ,刘英华 ,姚萍 ,梁平 ,刘志洋,李云义 【关键词】 血小板聚集 　　【Abstract】AIM： To investigate the changes of platelet adhesiveness (Padt), platelet aggregation (Pagt) and hemorheology following simulated pushpull maneuver in rats. METHODS： The rats were divided into control group, acceleration group and pushpull maneuver group. Placed in an animal centrifuge with body position reversing control system, the rats were exposed to +5 Gz 30 s →-5 Gz 3 s→+10 Gz 20 s in the pushpull maneuver group, +5 Gz 35 s→+10 Gz 20 s in the acceleration group respectively for 4 times at 5 min interval and in the control group the rats were placed in the same way for the same time but not exposed to ±Gz .The changes of PadT, PAgT and hemorheology were observed after repeated Gz exposures. RESULTS： Compared with the acceleration group, the PAdT and PAgT increased significantly in the simulated pushpull maneuver group ( 0.05 0.01). Theηb，ηrd and erythrocyte accumulation index (EAI) also increased ( 0.05). There were not significant differences in the abovementioned indexes between the control group and the acceleration group. CONCLUSION： The results suggest that repeated pushpull maneuver exposures 4 times can lead to the increase of PAdT, PAgT,ηb,ηrd and EAI. 　　【Keywords】acceleration；platelet adhesiveness platelet aggregation hemorheology pushpull maneuver pushpull effect 　　【摘要】 目的: 探讨模拟推拉动作后，大鼠血小板计数（PLT）、粘附（PAdT）、聚集功能（PAgT）和血液流变学的变化.方法: 将大鼠分为对照组、单纯加速度组和推拉动作组.利用动物离心机和体位翻转控制装置，推拉动作组暴露+5 Gz 30 s后， -5 Gz暴露3 s， +10 Gz暴露 20 s.单纯加速度组进行+5 Gz暴露 35 s后，+10 Gz暴露 20 s.共作用4次，两次之间间歇5 min.对照组只进行同样时间、同样方法的固定，但不受±Gz作用.观察模拟推拉动作后，PLT, PAdT,PAgT和血液流变学的变化.结果:推拉动作组与单纯加速度组相比，PAdT和PAgT都明显增加(005,001)；全血粘度（ηb）、全血还原粘度（ηrd）和红细胞聚集指数（EAI）亦增加（005）.单纯加速度组与对照组相比，各项指标差异无显著性.结论: 推拉动作 4次后，可引起PLT, PAdT和PAgT显著性增加，ηb,ηrd和EAI亦增加. 　　【关键词】 加速度；血小板粘附；血小板聚集；血液流变学；推拉动作；推拉效应 　　0引言 　　在小于+1 Gz的加速度后，迅速转入大于+1 Gz的飞行动作，叫做推拉动作.推拉动作使+Gz耐力下降的效应称为推拉效应［１］.由于+Gz耐力的下降，推拉动作时，在较低的G值情况下，即可发生GLOC，