水蛭对热刺激后血管壁增厚的干预作用_医学论文.docVIP

水蛭对热刺激后血管壁增厚的干预作用_医学论文 水蛭对热刺激后血管壁增厚的干预作用_医学论文 作者：屈 波，赵爱明，陈北阳，李迎秋 【摘要】 目的 探讨水蛭对热刺激后动脉管壁增厚的干预效果。方法 使用局部热刺激兔耳，温度为(43±0.5)℃，每次3h，每天1次，共做3次。模型组分别于造模后1周、2周、4周时取材；水蛭组实验前3天及实验后一直用水蛭灌胃，0.3g/(kg·d)，分别于造模后1周、2周及4周时取材。采用光学显微镜及图像分析仪进行形态学观察。结果 模型组热刺激后动脉中膜成分增殖并突入血管腔内，造成管腔变狭。 造模处平滑肌细胞层数及中膜厚度增加。水蛭组与模型组相比，增殖程度减小，各项观测指标均有不同程度的变化，但统计学分析差异无显著性。结论 0.3g/(kg·d)剂量的水蛭对热刺激后血管壁增厚的干预效果不理想。 【关键词】 动脉；狭窄；水蛭；形态学 【Abstract】 Objective This study was designed to build a new experimental model of proliferation of arterial smooth muscle cells(SMC).Methods The central artery of the rabbit ear was heated by(43±0.5)℃ for 3 hours a day，and for three days.We examined the morphological changes of the arterials by light microscopy and digital planimetry，3 days，1 week，2 weeks，and 4 weeks after experiment. Hirudo powder was administered 3 days before heating，and rabbits were examined 4 weeks after experiment.Results As the increasing of the time，the proliferation of SMC increased and extended to the lumine of the artery，but the internal elastic membrane was not disrupted. So，the lumina stenosis occuerd. Hirudo powder caused the proliferation of SMC to decrease. Although the lumina become smaller，the effect is not significant.Conclusion From the research results above，we can draw the conclusion that heat can induce arterial SMC to proliferate，and then decrease the area of the artery lumina. The model is available. Heat can activate autocrine growth of the local SMC，and alter the structures and function of vascular wall. originated from the host. Hirudo powder has a slight effect on decreacing SMC proliferaton，because it may not play a part in the relavent mechanism of heat. 【Key words】 artery； stenosis； Hirudo powder； morphology 水蛭是应用已久的活血祛瘀类药物，味咸苦，性平、有小毒，归肝经。其主要功效为破血逐瘀。临床广泛应用于心脑血栓性疾病及瘀血经闭的治疗中，亦可用于治疗生殖系统疾病如精不液化等。本实验研究水蛭对家兔热刺激后血管壁增厚的干预作用，现报告如下。 1 实验材料 1.1 实验动物 中国家兔48只，体重1.8～2.2kg，雄性，由湖南中医学院实验动物中心提供。合格证号为湘医动字第20-003号。 1.2 药物与试剂 水蛭购自湖南长沙九芝堂药店，产地为山东，宽体金线蛭，生产日期为2005年1月。 1.3 仪器 GSY型恒温水浴锅(北京医疗器械厂)；